Human KL ELISA Kit

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  • Alternative name

    Human klotho ELISA Kit;

  • Catalog
    E021912
  • species
    Human
  • GeneKL
  • Standard CurveHuman KL ELISA Kit
  • Other Species Human KL 6 ELISA KitHuman KL-6 ELISA KitMouse Kl ELISA KitMouse KL-6 ELISA KitMonkey KL ELISA KitRat Kl ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of KL. No significant cross-reactivity or interference between KL and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between KL and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman KL ELISA Kit allows for the in vitro quantitative determination of KL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Intended Uses: This KL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human KL. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||KL ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-KL antibody and an KL-HRP conjugate. The assay sample and buffer are incubated together with KL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the KL concentration since KL from samples and KL-HRP conjugate compete for the anti-KL antibody binding site. Since the number of sites is limited, as more sites are occupied by KL from the sample, fewer sites are left to bind KL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KL concentration in each sample is interpolated from this standard curve.
  • Human Klotho peptide Protein information
  • Uniprot ID KLOT_HUMAN
  • Uniprot AC Q9UEF7; Q5VZ95; Q96KV5; Q96KW5; Q9UEI9; Q9Y4F0;
  • UniGene Hs.524953;
  • GeneID 9365
  • KEGG hsa:9365;
  • Human Klotho peptide Protein SEQUENCE
  • SEQUENCE 1012 AA; 116181 MW; 62031BD73E322E63 CRC64;

    MPASAPPRRP RPPPPSLSLL LVLLGLGGRR LRAEPGDGAQ TWARFSRPPA

    PEAAGLFQGT FPDGFLWAVG SAAYQTEGGW QQHGKGASIW DTFTHHPLAP

    PGDSRNASLP LGAPSPLQPA TGDVASDSYN NVFRDTEALR ELGVTHYRFS

    ISWARVLPNG SAGVPNREGL RYYRRLLERL RELGVQPVVT LYHWDLPQRL

    QDAYGGWANR ALADHFRDYA ELCFRHFGGQ VKYWITIDNP YVVAWHGYAT

    GRLAPGIRGS PRLGYLVAHN LLLAHAKVWH LYNTSFRPTQ GGQVSIALSS

    HWINPRRMTD HSIKECQKSL DFVLGWFAKP VFIDGDYPES MKNNLSSILP

    DFTESEKKFI KGTADFFALC FGPTLSFQLL DPHMKFRQLE SPNLRQLLSW

    IDLEFNHPQI FIVENGWFVS GTTKRDDAKY MYYLKKFIME TLKAIKLDGV

    DVIGYTAWSL MDGFEWHRGY SIRRGLFYVD FLSQDKMLLP KSSALFYQKL

    IEKNGFPPLP ENQPLEGTFP CDFAWGVVDN YIQVDTTLSQ FTDLNVYLWD

    VHHSKRLIKV DGVVTKKRKS YCVDFAAIQP QIALLQEMHV THFRFSLDWA

    LILPLGNQSQ VNHTILQYYR CMASELVRVN ITPVVALWQP MAPNQGLPRL

    LARQGAWENP YTALAFAEYA RLCFQELGHH VKLWITMNEP YTRNMTYSAG

    HNLLKAHALA WHVYNEKFRH AQNGKISIAL QADWIEPACP FSQKDKEVAE

    RVLEFDIGWL AEPIFGSGDY PWVMRDWLNQ RNNFLLPYFT EDEKKLIQGT

    FDFLALSHYT TILVDSEKED PIKYNDYLEV QEMTDITWLN SPSQVAVVPW

    GLRKVLNWLK FKYGDLPMYI ISNGIDDGLH AEDDQLRVYY MQNYINEALK

    AHILDGINLC GYFAYSFNDR TAPRFGLYRY AADQFEPKAS MKHYRKIIDS

    NGFPGPETLE RFCPEEFTVC TECSFFHTRK SLLAFIAFLF FASIISLSLI

    FYYSKKGRRS YK

  • UCSC uc001uus.3; human. [Q9UEF7-1];



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