Human KL ELISA Kit

Alternative name
Human klotho ELISA Kit;
Catalog
E021912
species
Human
GeneKL
SpecificityThis assay has high sensitivity and excellent specificity for detection of KL. No significant cross-reactivity or interference between KL and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between KL and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman KL ELISA Kit allows for the in vitro quantitative determination of KL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalIntended Uses: This KL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human KL. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||KL ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-KL antibody and an KL-HRP conjugate. The assay sample and buffer are incubated together with KL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the KL concentration since KL from samples and KL-HRP conjugate compete for the anti-KL antibody binding site. Since the number of sites is limited, as more sites are occupied by KL from the sample, fewer sites are left to bind KL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KL concentration in each sample is interpolated from this standard curve.
Human Klotho peptide Protein information
Uniprot ID KLOT_HUMAN
Uniprot AC Q9UEF7; Q5VZ95; Q96KV5; Q96KW5; Q9UEI9; Q9Y4F0;
UniGene Hs.524953;
GeneID 9365
KEGG hsa:9365;
Human Klotho peptide Protein SEQUENCE
SEQUENCE 1012 AA; 116181 MW; 62031BD73E322E63 CRC64;
MPASAPPRRP RPPPPSLSLL LVLLGLGGRR LRAEPGDGAQ TWARFSRPPA
PEAAGLFQGT FPDGFLWAVG SAAYQTEGGW QQHGKGASIW DTFTHHPLAP
PGDSRNASLP LGAPSPLQPA TGDVASDSYN NVFRDTEALR ELGVTHYRFS
ISWARVLPNG SAGVPNREGL RYYRRLLERL RELGVQPVVT LYHWDLPQRL
QDAYGGWANR ALADHFRDYA ELCFRHFGGQ VKYWITIDNP YVVAWHGYAT
GRLAPGIRGS PRLGYLVAHN LLLAHAKVWH LYNTSFRPTQ GGQVSIALSS
HWINPRRMTD HSIKECQKSL DFVLGWFAKP VFIDGDYPES MKNNLSSILP
DFTESEKKFI KGTADFFALC FGPTLSFQLL DPHMKFRQLE SPNLRQLLSW
IDLEFNHPQI FIVENGWFVS GTTKRDDAKY MYYLKKFIME TLKAIKLDGV
DVIGYTAWSL MDGFEWHRGY SIRRGLFYVD FLSQDKMLLP KSSALFYQKL
IEKNGFPPLP ENQPLEGTFP CDFAWGVVDN YIQVDTTLSQ FTDLNVYLWD
VHHSKRLIKV DGVVTKKRKS YCVDFAAIQP QIALLQEMHV THFRFSLDWA
LILPLGNQSQ VNHTILQYYR CMASELVRVN ITPVVALWQP MAPNQGLPRL
LARQGAWENP YTALAFAEYA RLCFQELGHH VKLWITMNEP YTRNMTYSAG
HNLLKAHALA WHVYNEKFRH AQNGKISIAL QADWIEPACP FSQKDKEVAE
RVLEFDIGWL AEPIFGSGDY PWVMRDWLNQ RNNFLLPYFT EDEKKLIQGT
FDFLALSHYT TILVDSEKED PIKYNDYLEV QEMTDITWLN SPSQVAVVPW
GLRKVLNWLK FKYGDLPMYI ISNGIDDGLH AEDDQLRVYY MQNYINEALK
AHILDGINLC GYFAYSFNDR TAPRFGLYRY AADQFEPKAS MKHYRKIIDS
NGFPGPETLE RFCPEEFTVC TECSFFHTRK SLLAFIAFLF FASIISLSLI
FYYSKKGRRS YK
UCSC uc001uus.3; human. [Q9UEF7-1];

Category

Human KL ELISA Kit

Human klotho ELISA Kit;