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Alternative name
VEGF ELISA KIT; VEGF ELISA KIT; VEGF-EVEGF ELISA KIT;
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Catalog
E072371
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species
Mouse
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GeneVEGF-E
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Standard Curve
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SpecificityThis assay has high sensitivity and excellent specificity for detection of VEGF-E. No significant cross-reactivity or interference between VEGF-E and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between VEGF-E and all the analogues, therefore, cross reaction may still exist in some cases.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity1.0 pg/mL.
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Intended UseMouse VEGF-E ELISA Kit allows for the in vitro quantitative determination of VEGF-E , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyCancer
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Product Description
specificalPrinciple of the assay: VEGF-E ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-VEGF-E antibody and an VEGF-E-HRP conjugate. The assay sample and buffer are incubated together with VEGF-E-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the VEGF-E concentration since VEGF-E from samples and VEGF-E-HRP conjugate compete for the anti-VEGF-E antibody binding site. Since the number of sites is limited, as more sites are occupied by VEGF-E from the sample, fewer sites are left to bind VEGF-E-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VEGF-E concentration in each sample is interpolated from this standard curve.
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