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Human LIPF ELISA Kit

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  • Alternative name

    Human GL ELISA Kit;Human gastric lipase ELISA Kit;Human HGL ELISA Kit;Human HLAL ELISA Kit;Human lipase F, gastric type ELISA Kit;Human gastric triacylglycerol lipase ELISA Kit;Human lipase, gastric ELISA Kit;
  • Catalog
    E022784
  • species
    Human
  • GeneLIPF
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Lipase, Gastric (LIPF). No significant cross-reactivity or interference between Lipase, Gastric (LIPF) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.057ng/mL.
  • Intended UseHuman LIPF ELISA Kit allows for the in vitro quantitative determination of LIPF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipase, Gastric (LIPF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lipase, Gastric (LIPF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipase, Gastric (LIPF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lipase, Gastric (LIPF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Gastric triacylglycerol lipase Protein information
  • Uniprot ID LIPG_HUMAN
  • Uniprot AC P07098; B7Z723; F5H1P4; Q2M1P6; Q5VXI7; Q5VXI8; Q658L8;
  • UniGene Hs.523130;
  • GeneID 8513
  • KEGG hsa:8513;
  • Human Gastric triacylglycerol lipase Protein SEQUENCE

  • SEQUENCE 398 AA; 45238 MW; CD3EE1621C014F0F CRC64;

    MWLLLTMASL ISVLGTTHGL FGKLHPGSPE VTMNISQMIT YWGYPNEEYE

    VVTEDGYILE VNRIPYGKKN SGNTGQRPVV FLQHGLLASA TNWISNLPNN

    SLAFILADAG YDVWLGNSRG NTWARRNLYY SPDSVEFWAF SFDEMAKYDL

    PATIDFIVKK TGQKQLHYVG HSQGTTIGFI AFSTNPSLAK RIKTFYALAP

    VATVKYTKSL INKLRFVPQS LFKFIFGDKI FYPHNFFDQF LATEVCSREM

    LNLLCSNALF IICGFDSKNF NTSRLDVYLS HNPAGTSVQN MFHWTQAVKS

    GKFQAYDWGS PVQNRMHYDQ SQPPYYNVTA MNVPIAVWNG GKDLLADPQD

    VGLLLPKLPN LIYHKEIPFY NHLDFIWAMD APQEVYNDIV SMISEDKK

  • UCSC uc001kfg.3; human. [P07098-1];



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