Human Alpha GAL ELISA Kit

Contact Us
  • Catalog
  • species
  • GeneAlpha GAL
  • Other Species Human alpha-GAL ELISA KitMouse alpha-Gal ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of alpha-Galactosidase. No significant cross-reactivity or interference between alpha-Galactosidase and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between alpha-Galactosidase and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman Alpha GAL ELISA Kit allows for the in vitro quantitative determination of Alpha GAL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Intended Uses: This alpha-Galactosidase ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human alpha-Galactosidase. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||Alpha-Galactosidase ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-alpha-Galactosidase antibody and an alpha-Galactosidase-HRP conjugate. The assay sample and buffer are incubated together with alpha-Galactosidase-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the alpha-Galactosidase concentration since alpha-Galactosidase from samples and alpha-Galactosidase-HRP conjugate compete for the anti-alpha-Galactosidase antibody binding site. Since the number of sites is limited, as more sites are occupied by alpha-Galactosidase from the sample, fewer sites are left to bind alpha-Galactosidase-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The alpha-Galactosidase concentration in each sample is interpolated from this standard curve.

Inquiry About Human Alpha GAL ELISA Kit If you hope to order it or contact us directly, please contact us via We will get back to you in 12hrs,Thanks!