SpecificityThis assay recognizes recombinant and natural mouse Uncharacterized protein C13orf33 homolog. No significant cross-reactivity or interference was observed.
Intended UseMouse Awms3 ELISA Kit allows for the in vitro quantitative determination of Awms3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Uncharacterized protein C13orf33 homolog. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Uncharacterized protein C13orf33 homolog and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Uncharacterized protein C13orf33 homolog, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Uncharacterized protein C13orf33 homolog in the samples is then determined by comparing the O.D. of the samples to the standard curve.
No manual or datasheet available for this product.