Human PRKG2 ELISA Kit

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  • Alternative name

    Human cGK 2 ELISA Kit;Human cGK2 ELISA Kit;Human cGMP-dependent protein kinase II ELISA Kit;Human cGKII ELISA Kit;Human PRKGR2 ELISA Kit;Human protein kinase, cGMP-dependent, type II ELISA Kit;Human cGMP-dependent protein kinase 2 ELISA Kit;Human testicular tissue protein Li 99 ELISA Kit;

  • Catalog
    E031658
  • species
    Human
  • GenePRKG2
  • Standard CurveHuman PRKG2 ELISA Kit
  • Other Species Mouse Prkg2 ELISA KitRat Prkg2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Protein Kinase, cGMP Dependent Type II (PRKG2). No significant cross-reactivity or interference between Protein Kinase, cGMP Dependent Type II (PRKG2) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.127ng/mL.
  • Intended UseHuman PRKG2 ELISA Kit allows for the in vitro quantitative determination of PRKG2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase, cGMP Dependent Type II (PRKG2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase, cGMP Dependent Type II (PRKG2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase, cGMP Dependent Type II (PRKG2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase, cGMP Dependent Type II (PRKG2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human cGMP-dependent protein kinase 2 Protein information
  • Uniprot ID KGP2_HUMAN
  • Uniprot AC Q13237; B4DMX3; E7EPE6; O00125; O60916;
  • UniGene Hs.232044; Hs.570833; Hs.739943;
  • GeneID 5593
  • KEGG hsa:5593;
  • Human cGMP-dependent protein kinase 2 Protein SEQUENCE
  • SEQUENCE 762 AA; 87432 MW; FA7BA149906B5996 CRC64;

    MGNGSVKPKH SKHPDGHSGN LTTDALRNKV TELERELRRK DAEIQEREYH

    LKELREQLSK QTVAIAELTE ELQNKCIQLN KLQDVVHMQG GSPLQASPDK

    VPLEVHRKTS GLVSLHSRRG AKAGVSAEPT TRTYDLNKPP EFSFEKARVR

    KDSSEKKLIT DALNKNQFLK RLDPQQIKDM VECMYGRNYQ QGSYIIKQGE

    PGNHIFVLAE GRLEVFQGEK LLSSIPMWTT FGELAILYNC TRTASVKAIT

    NVKTWALDRE VFQNIMRRTA QARDEQYRNF LRSVSLLKNL PEDKLTKIID

    CLEVEYYDKG DYIIREGEEG STFFILAKGK VKVTQSTEGH DQPQLIKTLQ

    KGEYFGEKAL ISDDVRSANI IAEENDVACL VIDRETFNQT VGTFEELQKY

    LEGYVANLNR DDEKRHAKRS MSNWKLSKAL SLEMIQLKEK VARFSSSSPF

    QNLEIIATLG VGGFGRVELV KVKNENVAFA MKCIRKKHIV DTKQQEHVYS

    EKRILEELCS PFIVKLYRTF KDNKYVYMLL EACLGGELWS ILRDRGSFDE

    PTSKFCVACV TEAFDYLHRL GIIYRDLKPE NLILDAEGYL KLVDFGFAKK

    IGSGQKTWTF CGTPEYVAPE VILNKGHDFS VDFWSLGILV YELLTGNPPF

    SGVDQMMTYN LILKGIEKMD FPRKITRRPE DLIRRLCRQN PTERLGNLKN

    GINDIKKHRW LNGFNWEGLK ARSLPSPLQR ELKGPIDHSY FDKYPPEKGM

    PPDELSGWDK DF

  • UCSC uc003hmh.3; human. [Q13237-1];


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