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Alternative name
CC chemokine ABCD-2 ELISA KIT; CC chemokine ABCD-2 ELISA KIT; Chemokine (C-C motif) ligand 17 ELISA KIT; Thymus and activation-regulated chemokineCcl17 ELISA KIT; ABCD-2 ELISA KIT; TARC ELISA KIT; mCG_12286 ELISA KIT
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Catalog
E070116
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species
Mouse
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GeneCCL17
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SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse TARC. No significant cross-reactivity or interference between mouse TARC and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe minimum detectable dose of mouse TARC is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
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Intended UseMouse CCL17 ELISA Kit allows for the in vitro quantitative determination of CCL17 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TARC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TARC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TARC is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TARC bound in the initial step. The color development is stopped and the intensity of the color is measured.
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