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Human SAE1 ELISA Kit

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  • Alternative name

    Human ubiquitin-like 1-activating enzyme E1A ELISA Kit;Human AOS1 ELISA Kit;Human SUA1 ELISA Kit;Human UBLE1A ELISA Kit;Human HSPC140 ELISA Kit;Human SUMO1 activating enzyme subunit 1 ELISA Kit;Human SUMO-activating enzyme subunit 1 ELISA Kit;Human SUMO-1 activating enzyme E1 N subunit ELISA Kit;Human SUMO-1 activating enzyme subunit 1 ELISA Kit;Human activator of SUMO1 ELISA Kit;Human sentrin/SUMO-activating protein AOS1 ELISA Kit;Human ubiquitin-like protein SUMO-1 activating enzyme ELISA Kit;
  • Catalog
    E036911
  • species
    Human
  • GeneSAE1
  • Other Species Mouse Sae1 ELISA KitBovine SAE1 ELISA KitRat Sae1 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.094ng/ml
  • Intended UseHuman SAE1 ELISA Kit allows for the in vitro quantitative determination of SAE1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human SAE1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human SAE1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human SAE1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human SAE1 can be calculated.
  • Human SUMO-activating enzyme subunit 1, N-terminally processed Protein information
  • Uniprot ID SAE1_HUMAN
  • Uniprot AC Q9UBE0; B2RDP5; B3KMQ2; F5GXX7; G3XAK6; O95717; Q9P020;
  • UniGene Hs.515500;
  • GeneID 10055
  • KEGG hsa:10055;
  • Human SUMO-activating enzyme subunit 1, N-terminally processed Protein SEQUENCE

  • SEQUENCE 346 AA; 38450 MW; E2B10A69FF2ED746 CRC64;

    MVEKEEAGGG ISEEEAAQYD RQIRLWGLEA QKRLRASRVL LVGLKGLGAE

    IAKNLILAGV KGLTMLDHEQ VTPEDPGAQF LIRTGSVGRN RAEASLERAQ

    NLNPMVDVKV DTEDIEKKPE SFFTQFDAVC LTCCSRDVIV KVDQICHKNS

    IKFFTGDVFG YHGYTFANLG EHEFVEEKTK VAKVSQGVED GPDTKRAKLD

    SSETTMVKKK VVFCPVKEAL EVDWSSEKAK AALKRTTSDY FLLQVLLKFR

    TDKGRDPSSD TYEEDSELLL QIRNDVLDSL GISPDLLPED FVRYCFSEMA

    PVCAVVGGIL AQEIVKALSQ RDPPHNNFFF FDGMKGNGIV ECLGPK

  • UCSC uc002pgc.4; human. [Q9UBE0-1];


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