Human ENG ELISA Kit

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  • Alternative name

    Human END ELISA Kit;Human HHT1 ELISA Kit;Human ORW1 ELISA Kit;Human endoglin ELISA Kit;Human CD105 antigen ELISA Kit;

  • Catalog
    E007263
  • species
    Human
  • GeneENG
  • Standard CurveHuman ENG ELISA Kit
  • Other Species Human ENG/sCD105 ELISA KitMouse Eng ELISA KitMouse ENG/sCD105 ELISA KitPorcine ENG ELISA Kit
  • SpecificityNatural and recombinant human CD105
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 15pg/ml
  • Intended UseHuman ENG ELISA Kit allows for the in vitro quantitative determination of ENG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: human CD105 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD105 has been precoated onto 96-well plates. Standards(CHO,E26-G586) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD105 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD105 amount of sample captured in plate. Background: CD105, also called Endoglin, is a homodimeric membrane glycoprotein primarily associated with human vascular endothelium. It is also found on bone marrow proerythroblasts, activated monocytes, and lymphoblasts in childhood leukemia. Endoglin is a component of the transforming growth factor-beta (TGFB) receptor complex and binds TGFB1 with high affinity.1 CD105 gene is mapped to 9q34.1. The coding region of the gene contains 14 exons.2 Theprotein consists of a homodimer of 180 kDA with disulfide links. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Its expression is regulated during heart development. Furthermore, it also has a role in the balance of ALK1 and ALK5 signaling to regulate endothelial cell proliferation.3Moreover, the elevated expression of endoglin in the surgically excised CNVMs suggested a persisting postmitotic activation in an advanced stage of neovascular tissue.4 The standard product used in this kit is extracellular part ofrecombinant human CD105, from E26 to G586. As a result of glycosylation, the molecular mass is 75-85KDa.
  • Human Endoglin Protein information
  • Uniprot ID EGLN_HUMAN
  • Uniprot AC P17813; Q14248; Q14926; Q5T9C0;
  • UniGene Hs.76753;
  • GeneID 2022
  • KEGG hsa:2022;
  • Human Endoglin Protein SEQUENCE
  • SEQUENCE 658 AA; 70578 MW; 49CA2CE013298D17 CRC64;

    MDRGTLPLAV ALLLASCSLS PTSLAETVHC DLQPVGPERG EVTYTTSQVS

    KGCVAQAPNA ILEVHVLFLE FPTGPSQLEL TLQASKQNGT WPREVLLVLS

    VNSSVFLHLQ ALGIPLHLAY NSSLVTFQEP PGVNTTELPS FPKTQILEWA

    AERGPITSAA ELNDPQSILL RLGQAQGSLS FCMLEASQDM GRTLEWRPRT

    PALVRGCHLE GVAGHKEAHI LRVLPGHSAG PRTVTVKVEL SCAPGDLDAV

    LILQGPPYVS WLIDANHNMQ IWTTGEYSFK IFPEKNIRGF KLPDTPQGLL

    GEARMLNASI VASFVELPLA SIVSLHASSC GGRLQTSPAP IQTTPPKDTC

    SPELLMSLIQ TKCADDAMTL VLKKELVAHL KCTITGLTFW DPSCEAEDRG

    DKFVLRSAYS SCGMQVSASM ISNEAVVNIL SSSSPQRKKV HCLNMDSLSF

    QLGLYLSPHF LQASNTIEPG QQSFVQVRVS PSVSEFLLQL DSCHLDLGPE

    GGTVELIQGR AAKGNCVSLL SPSPEGDPRF SFLLHFYTVP IPKTGTLSCT

    VALRPKTGSQ DQEVHRTVFM RLNIISPDLS GCTSKGLVLP AVLGITFGAF

    LIGALLTAAL WYIYSHTRSP SKREPVVAVA APASSESSST NHSIGSTQST

    PCSTSSMA

  • UCSC uc004bsj.6; human. [P17813-1];



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