SpecificityThis assay has high sensitivity and excellent specificity for detection of CAMP. No significant cross-reactivity or interference between CAMP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CAMP and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseHuman CAMP ELISA Kit allows for the in vitro quantitative determination of CAMP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the Assay: CAMP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CAMP antibody and an CAMP-HRP conjugate. The assay sample and buffer are incubated together with CAMP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CAMP concentration since CAMP from samples and CAMP-HRP conjugate compete for the anti-CAMP antibody binding site. Since the number of sites is limited, as more sites are occupied by CAMP from the sample, fewer sites are left to bind CAMP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CAMP concentration in each sample is interpolated from this standard curve.
Human Antibacterial peptide LL-37 Protein information
Human Antibacterial peptide LL-37 Protein SEQUENCE