Mouse Igf2 ELISA Kit

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  • Alternative name

    Mouse Igf-II ELISA Kit;Mouse multiplication-stimulating polypeptide ELISA Kit;Mouse Igf-2 ELISA Kit;Mouse AL033362 ELISA Kit;Mouse M6pr ELISA Kit;Mouse Mpr ELISA Kit;Mouse Peg2 ELISA Kit;Mouse insulin-like growth factor 2 ELISA Kit;Mouse insulin-like growth factor II ELISA Kit;

  • Catalog
    E056227
  • species
    Mouse
  • GeneIgf2
  • Standard CurveMouse Igf2 ELISA Kit
  • Other Species Human IGF2 ELISA KitHuman IGF2-AS ELISA KitBovine IGF2 ELISA KitGuinea Pig IGF2 ELISA KitChicken IGF2 ELISA KitHorse IGF2 ELISA KitPorcine IGF2 ELISA KitRat Igf2 ELISA KitSheep IGF2 ELISA KitHuman INS-IGF2 ELISA Kit
  • SpecificityNatural and recombinant mouse IGF-2
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 5pg/ml
  • Intended UseMouse Igf2 ELISA Kit allows for the in vitro quantitative determination of Igf2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: mouse IGF-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IGF-2 has been precoated onto 96-well plates. Standards(E.coli,A25-E91) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGF-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IGF-2 amount of sample captured in plate. Background: Insulin-like growth factor II is also known as somatomedin A. IGF-2 is a member of the insulin family of polypeptide growth factors that is involved in development and growth.1 It is paternally expressed in the fetus and placenta.2IGF-II is a mitogen for many cell types and an important modulator of muscle growth and differentiation. IGF-II gene is prevalently expressed during prenatal development and its gene activity is regulated by genomic imprinting, in that the allele inherited from the father is active and the allele inherited from the mother is inactive in most normal tissues.3 IGF-II appears to be induced by placental lactogen during prenatal development.4 It is a mediator ofprolactin-induced alveologenesis; prolactin, IGF-2, and cyclin D1, all of which are overexpressed in breast cancers, are components of a developmental pathway in the mammary gland.5
  • Mouse Preptin Protein information
  • Uniprot ID IGF2_MOUSE
  • Uniprot AC P09535;
  • UniGene Mm.3862;
  • GeneID 16002
  • KEGG mmu:16002;
  • Mouse Preptin Protein SEQUENCE
  • SEQUENCE 180 AA; 20030 MW; 01730F8856EE6D7B CRC64;

    MGIPVGKSML VLLISLAFAL CCIAAYGPGE TLCGGELVDT LQFVCSDRGF

    YFSRPSSRAN RRSRGIVEEC CFRSCDLALL ETYCATPAKS ERDVSTSQAV

    LPDDFPRYPV GKFFQYDTWR QSAGRLRRGL PALLRARRGR MLAKELKEFR

    EAKRHRPLIV LPPKDPAHGG ASSEMSSNHQ

  • UCSC uc009kod.2; mouse.;




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