Mouse GAL7 ELISA Kit

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  • Catalog
    E053801
  • species
    Mouse
  • GeneGAL7
  • Other Species Human GAL7 ELISA KitChicken GAL7 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.094ng/ml
  • Intended UseMouse GAL7 ELISA Kit allows for the in vitro quantitative determination of GAL7 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse GAL7 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse GAL7 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse GAL7 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse GAL7 can be calculated.




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