Human CLDN6 ELISA Kit

Alternative name
Human skullin ELISA Kit;Human claudin 6 ELISA Kit;Human claudin-6 ELISA Kit;
Catalog
E009608
species
Human
GeneCLDN6
SpecificityThis assay has high sensitivity and excellent specificity for detection of CLDN6. No significant cross-reactivity or interference between CLDN6 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CLDN6 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CLDN6 ELISA Kit allows for the in vitro quantitative determination of CLDN6 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalIntended Uses: This CLDN6 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CLDN6. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: CLDN6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-CLDN6 antibody and an CLDN6-HRP conjugate. The assay sample and buffer are incubated together with CLDN6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLDN6 concentration since CLDN6 from samples and CLDN6-HRP conjugate compete for the anti-CLDN6 antibody binding site. Since the number of sites is limited, as more sites are occupied by CLDN6 from the sample, fewer sites are left to bind CLDN6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLDN6 concentration in each sample is interpolated from this standard curve.
Human Claudin-6 Protein information
Uniprot ID CLD6_HUMAN
Uniprot AC P56747; B3KQP9; D3DUA5;
UniGene Hs.533779;
GeneID 9074
KEGG hsa:9074;
Human Claudin-6 Protein SEQUENCE
SEQUENCE 220 AA; 23292 MW; D80AECB5681502AA CRC64;
MASAGMQILG VVLTLLGWVN GLVSCALPMW KVTAFIGNSI VVAQVVWEGL
WMSCVVQSTG QMQCKVYDSL LALPQDLQAA RALCVIALLV ALFGLLVYLA
GAKCTTCVEE KDSKARLVLT SGIVFVISGV LTLIPVCWTA HAIIRDFYNP
LVAEAQKREL GASLYLGWAA SGLLLLGGGL LCCTCPSGGS QGPSHYMARY
STSAPAISRG PSEYPTKNYV
UCSC uc002csu.5; human.;

Human CLDN6 ELISA Kit

Human skullin ELISA Kit;Human claudin 6 ELISA Kit;Human claudin-6 ELISA Kit;

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