Human CLDN6 ELISA Kit

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  • Alternative name

    Human skullin ELISA Kit;Human claudin 6 ELISA Kit;Human claudin-6 ELISA Kit;

  • Catalog
    E009608
  • species
    Human
  • GeneCLDN6
  • Standard CurveHuman CLDN6 ELISA Kit
  • Other Species Mouse Cldn6 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CLDN6. No significant cross-reactivity or interference between CLDN6 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CLDN6 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman CLDN6 ELISA Kit allows for the in vitro quantitative determination of CLDN6 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Intended Uses: This CLDN6 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CLDN6. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: CLDN6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-CLDN6 antibody and an CLDN6-HRP conjugate. The assay sample and buffer are incubated together with CLDN6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLDN6 concentration since CLDN6 from samples and CLDN6-HRP conjugate compete for the anti-CLDN6 antibody binding site. Since the number of sites is limited, as more sites are occupied by CLDN6 from the sample, fewer sites are left to bind CLDN6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLDN6 concentration in each sample is interpolated from this standard curve.
  • Human Claudin-6 Protein information
  • Uniprot ID CLD6_HUMAN
  • Uniprot AC P56747; B3KQP9; D3DUA5;
  • UniGene Hs.533779;
  • GeneID 9074
  • KEGG hsa:9074;
  • Human Claudin-6 Protein SEQUENCE
  • SEQUENCE 220 AA; 23292 MW; D80AECB5681502AA CRC64;

    MASAGMQILG VVLTLLGWVN GLVSCALPMW KVTAFIGNSI VVAQVVWEGL

    WMSCVVQSTG QMQCKVYDSL LALPQDLQAA RALCVIALLV ALFGLLVYLA

    GAKCTTCVEE KDSKARLVLT SGIVFVISGV LTLIPVCWTA HAIIRDFYNP

    LVAEAQKREL GASLYLGWAA SGLLLLGGGL LCCTCPSGGS QGPSHYMARY

    STSAPAISRG PSEYPTKNYV

  • UCSC uc002csu.5; human.;



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