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Mouse Mtor ELISA Kit

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  • Alternative name

    Mouse FK506-binding protein 12-rapamycin complex-associated protein 1 ELISA Kit;Mouse FKBP12-rapamycin complex-associated protein ELISA Kit;Mouse mammalian target of rapamycin ELISA Kit;Mouse mTOR ELISA Kit;Mouse Mechanistic target of rapamycin ELISA Kit;Mouse rapamycin target protein 1 ELISA Kit;Mouse RAPT1 ELISA Kit;Mouse FRAP ELISA Kit;Mouse Frap1 ELISA Kit;Mouse 2610315D21Rik ELISA Kit;Mouse AI327068 ELISA Kit;Mouse FRAP2 ELISA Kit;Mouse RAFT1 ELISA Kit;Mouse flat ELISA Kit;Mouse mechanistic target of rapamycin (serine/threonine kinase) ELISA Kit;Mouse serine/threonine-protein kinase mTOR ELISA Kit;Mouse FK506 binding protein 12-rapamycin associated protein 1 ELISA Kit;Mouse FKBP-rapamycin associated protein (FRAP) ELISA Kit;Mouse angiopoietin-like factor CDT6 ELISA Kit;
  • Catalog
    E059429
  • species
    Mouse
  • GeneMtor
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of mTOR. No significant cross-reactivity or interference between mTOR and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between mTOR and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse Mtor ELISA Kit allows for the in vitro quantitative determination of Mtor , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Intended Uses: This mTOR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse mTOR. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||MTOR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-mTOR antibody and an mTOR-HRP conjugate. The assay sample and buffer are incubated together with mTOR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the mTOR concentration since mTOR from samples and mTOR-HRP conjugate compete for the anti-mTOR antibody binding site. Since the number of sites is limited, as more sites are occupied by mTOR from the sample, fewer sites are left to bind mTOR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The mTOR concentration in each sample is interpolated from this standard curve.
  • Mouse Serine/threonine-protein kinase mTOR Protein information
  • Uniprot ID MTOR_MOUSE
  • Uniprot AC Q9JLN9; Q2KHT0; Q811J5; Q9CST1;
  • UniGene Mm.21158;
  • GeneID 56717
  • KEGG mmu:56717;
  • Mouse Serine/threonine-protein kinase mTOR Protein SEQUENCE

  • SEQUENCE 2549 AA; 288789 MW; 56302E5171FB6DBD CRC64;

    MLGTGPAVAT ASAATSSNVS VLQQFASGLK SRNEETRAKA AKELQHYVTM

    ELREMSQEES TRFYDQLNHH IFELVSSSDA NERKGGILAI ASLIGVEGGN

    STRIGRFANY LRNLLPSSDP VVMEMASKAI GRLAMAGDTF TAEYVEFEVK

    RALEWLGADR NEGRRHAAVL VLRELAISVP TFFFQQVQPF FDNIFVAVWD

    PKQAIREGAV AALRACLILT TQREPKEMQK PQWYRHTFEE AEKGFDETLA

    KEKGMNRDDR IHGALLILNE LVRISSMEGE RLREEMEEIT QQQLVHDKYC

    KDLMGFGTKP RHITPFTSFQ AVQPQQPNAL VGLLGYSSPQ GLMGFGTSPS

    PAKSTLVESR CCRDLMEEKF DQVCQWVLKC RSSKNSLIQM TILNLLPRLA

    AFRPSAFTDT QYLQDTMNHV LSCVKKEKER TAAFQALGLL SVAVRSEFKV

    YLPRVLDIIR AALPPKDFAH KRQKTVQVDA TVFTCISMLA RAMGPGIQQD

    IKELLEPMLA VGLSPALTAV LYDLSRQIPQ LKKDIQDGLL KMLSLVLMHK

    PLRHPGMPKG LAHQLASPGL TTLPEASDVA SITLALRTLG SFEFEGHSLT

    QFVRHCADHF LNSEHKEIRM EAARTCSRLL TPSIHLISGH AHVVSQTAVQ

    VVADVLSKLL VVGITDPDPD IRYCVLASLD ERFDAHLAQA ENLQALFVAL

    NDQVFEIREL AICTVGRLSS MNPAFVMPFL RKMLIQILTE LEHSGIGRIK

    EQSARMLGHL VSNAPRLIRP YMEPILKALI LKLKDPDPDP NPGVINNVLA

    TIGELAQVSG LEMRKWVDEL FIIIMDMLQD SSLLAKRQVA LWTLGQLVAS

    TGYVVEPYRK YPTLLEVLLN FLKTEQNQGT RREAIRVLGL LGALDPYKHK

    VNIGMIDQSR DASAVSLSES KSSQDSSDYS TSEMLVNMGN LPLDEFYPAV

    SMVALMRIFR DQSLSHHHTM VVQAITFIFK SLGLKCVQFL PQVMPTFLNV

    IRVCDGAIRE FLFQQLGMLV SFVKSHIRPY MDEIVTLMRE FWVMNTSIQS

    TIILLIEQIV VALGGEFKLY LPQLIPHMLR VFMHDNSQGR IVSIKLLAAI

    QLFGANLDDY LHLLLPPIVK LFDAPEVPLP SRKAALETVD RLTESLDFTD

    YASRIIHPIV RTLDQSPELR STAMDTLSSL VFQLGKKYQI FIPMVNKVLV

    RHRINHQRYD VLICRIVKGY TLADEEEDPL IYQHRMLRSS QGDALASGPV

    ETGPMKKLHV STINLQKAWG AARRVSKDDW LEWLRRLSLE LLKDSSSPSL

    RSCWALAQAY NPMARDLFNA AFVSCWSELN EDQQDELIRS IELALTSQDI

    AEVTQTLLNL AEFMEHSDKG PLPLRDDNGI VLLGERAAKC RAYAKALHYK

    ELEFQKGPTP AILESLISIN NKLQQPEAAS GVLEYAMKHF GELEIQATWY

    EKLHEWEDAL VAYDKKMDTN KEDPELMLGR MRCLEALGEW GQLHQQCCEK

    WTLVNDETQA KMARMAAAAA WGLGQWDSME EYTCMIPRDT HDGAFYRAVL

    ALHQDLFSLA QQCIDKARDL LDAELTAMAG ESYSRAYGAM VSCHMLSELE

    EVIQYKLVPE RREIIRQIWW ERLQGCQRIV EDWQKILMVR SLVVSPHEDM

    RTWLKYASLC GKSGRLALAH KTLVLLLGVD PSRQLDHPLP TAHPQVTYAY

    MKNMWKSARK IDAFQHMQHF VQTMQQQAQH AIATEDQQHK QELHKLMARC

    FLKLGEWQLN LQGINESTIP KVLQYYSAAT EHDRSWYKAW HAWAVMNFEA

    VLHYKHQNQA RDEKKKLRHA SGANITNATT AATTAASAAA ATSTEGSNSE

    SEAESNENSP TPSPLQKKVT EDLSKTLLLY TVPAVQGFFR SISLSRGNNL

    QDTLRVLTLW FDYGHWPDVN EALVEGVKAI QIDTWLQVIP QLIARIDTPR

    PLVGRLIHQL LTDIGRYHPQ ALIYPLTVAS KSTTTARHNA ANKILKNMCE

    HSNTLVQQAM MVSEELIRVA ILWHEMWHEG LEEASRLYFG ERNVKGMFEV

    LEPLHAMMER GPQTLKETSF NQAYGRDLME AQEWCRKYMK SGNVKDLTQA

    WDLYYHVFRR ISKQLPQLTS LELQYVSPKL LMCRDLELAV PGTYDPNQPI

    IRIQSIAPSL QVITSKQRPR KLTLMGSNGH EFVFLLKGHE DLRQDERVMQ

    LFGLVNTLLA NDPTSLRKNL SIQRYAVIPL STNSGLIGWV PHCDTLHALI

    RDYREKKKIL LNIEHRIMLR MAPDYDHLTL MQKVEVFEHA VNNTAGDDLA

    KLLWLKSPSS EVWFDRRTNY TRSLAVMSMV GYILGLGDRH PSNLMLDRLS

    GKILHIDFGD CFEVAMTREK FPEKIPFRLT RMLTNAMEVT GLDGNYRTTC

    HTVMEVLREH KDSVMAVLEA FVYDPLLNWR LMDTNTKGNK RSRTRTDSYS

    AGQSVEILDG VELGEPAHKK AGTTVPESIH SFIGDGLVKP EALNKKAIQI

    INRVRDKLTG RDFSHDDTLD VPTQVELLIK QATSHENLCQ CYIGWCPFW

  • UCSC uc008vur.2; mouse. [Q9JLN9-1];



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