Human LIMK1 ELISA Kit

Alternative name
Human LIMK-1 ELISA Kit;Human LIMK ELISA Kit;Human LIM domain kinase 1 ELISA Kit;Human LIM motif-containing protein kinase ELISA Kit;
Catalog
E022715
species
Human
GeneLIMK1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human LIMK1. No significant cross-reactivity or interference between Human LIMK1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity7.8 pg/ml
Intended UseHuman LIMK1 ELISA Kit allows for the in vitro quantitative determination of LIMK1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LIMK1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for LIMK1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain LIMK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of LIMK1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human LIM domain kinase 1 Protein information
Uniprot ID LIMK1_HUMAN
Uniprot AC Q9Y5Q1;
UniGene Hs.647035;
GeneID 3984
KEGG hsa:3984;
Human LIM domain kinase 1 Protein SEQUENCE
SEQUENCE 647 AA; 72585 MW; 9838BEFBE7006447 CRC64;
MRLTLLCCTW REERMGEEGS ELPVCASCGQ RIYDGQYLQA LNADWHADCF
RCCDCSASLS HQYYEKDGQL FCKKDYWARY GESCHGCSEQ ITKGLVMVAG
ELKYHPECFI CLTCGTFIGD GDTYTLVEHS KLYCGHCYYQ TVVTPVIEQI
LPDSPGSHLP HTVTLVSIPA SSHGKRGLSV SIDPPHGPPG CGTEHSHTVR
VQGVDPGCMS PDVKNSIHVG DRILEINGTP IRNVPLDEID LLIQETSRLL
QLTLEHDPHD TLGHGLGPET SPLSSPAYTP SGEAGSSARQ KPVLRSCSID
RSPGAGSLGS PASQRKDLGR SESLRVVCRP HRIFRPSDLI HGEVLGKGCF
GQAIKVTHRE TGEVMVMKEL IRFDEETQRT FLKEVKVMRC LEHPNVLKFI
GVLYKDKRLN FITEYIKGGT LRGIIKSMDS QYPWSQRVSF AKDIASGMAY
LHSMNIIHRD LNSHNCLVRE NKNVVVADFG LARLMVDEKT QPEGLRSLKK
PDRKKRYTVV GNPYWMAPEM INGRSYDEKV DVFSFGIVLC EIIGRVNADP
DYLPRTMDFG LNVRGFLDRY CPPNCPPSFF PITVRCCDLD PEKRPSFVKL
EHWLETLRMH LAGHLPLGPQ LEQLDRGFWE TYRRGESGLP AHPEVPD
UCSC uc003uaa.3; human. [P53667-1];

Category

Human LIMK1 ELISA Kit

Human LIMK-1 ELISA Kit;Human LIMK ELISA Kit;Human LIM domain kinase 1 ELISA Kit;Human LIM motif-containing protein kinase ELISA Kit;