Human PKBg ELISA Kit

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  • Alternative name

    RAC-gamma serine/threonine-protein kinase ELISA KIT; Protein kinase Akt-3 ELISA KIT; Protein kinase B gamma ELISA KIT; PKB gamma ELISA KIT; RAC-PK-gamma ELISA KIT; STK-2AKT3 ELISA KIT; PKBG ELISA KIT; PKB gamma ELISA KIT

  • Catalog
    E031578
  • species
    Human
  • GenePKBg
  • Standard CurveHuman PKBg ELISA Kit
  • Other Species Mouse PKBG ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of PKBgamma. No significant cross-reactivity or interference between PKBgamma and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PKBgamma and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman PKBg ELISA Kit allows for the in vitro quantitative determination of PKBg , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Principle of the assay: PKBgamma ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PKBgamma antibody and an PKBgamma-HRP conjugate. The assay sample and buffer are incubated together with PKBgamma-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PKBgamma concentration since PKBgamma from samples and PKBgamma-HRP conjugate compete for the anti-PKBgamma antibody binding site. Since the number of sites is limited, as more sites are occupied by PKBgamma from the sample, fewer sites are left to bind PKBgamma-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PKBgamma concentration in each sample is interpolated from this standard curve.



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