Human HSPB1 ELISA Kit

Alternative name
Human HspB1 ELISA Kit;Human 28 kDa heat shock protein ELISA Kit;Human estrogen-regulated 24 kDa protein ELISA Kit;Human heat shock 27 kDa protein ELISA Kit;Human HSP 27 ELISA Kit;Human stress-responsive protein 27 ELISA Kit;Human SRP27 ELISA Kit;Human HSP27 ELISA Kit;Human HSP28 ELISA Kit;Human CMT2F ELISA Kit;Human HEL-S-102 ELISA Kit;Human HMN2B ELISA Kit;Human HS.76067 ELISA Kit;Human Hsp25 ELISA Kit;Human heat shock protein family B (small) member 1 ELISA Kit;Human heat shock protein beta-1 ELISA Kit;Human epididymis secretory protein Li 102 ELISA Kit;Human heat shock 27kD protein 1 ELISA Kit;Human heat shock 27kDa protein 1 ELISA Kit;
Catalog
E017932
species
Human
GeneHSPB1
SpecificityThis assay has high sensitivity and excellent specificity for detection of human HSP-27. No significant cross-reactivity or interference between human HSP-27 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human HSP-27 is typically less than 0.312 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the
Intended UseHuman HSPB1 ELISA Kit allows for the in vitro quantitative determination of HSPB1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HSP-27 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HSP-27 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HSP-27 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HSP-27 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Heat shock protein beta-1 Protein information
Uniprot ID HSPB1_HUMAN
Uniprot AC Q9UC36;
UniGene Hs.520973;
GeneID 3315
KEGG hsa:3315;
Human Heat shock protein beta-1 Protein SEQUENCE
SEQUENCE 205 AA; 22783 MW; 1B4DC44A6F6606D5 CRC64;
MTERRVPFSL LRGPSWDPFR DWYPHSRLFD QAFGLPRLPE EWSQWLGGSS
WPGYVRPLPP AAIESPAVAA PAYSRALSRQ LSSGVSEIRH TADRWRVSLD
VNHFAPDELT VKTKDGVVEI TGKHEERQDE HGYISRCFTR KYTLPPGVDP
TQVSSSLSPE GTLTVEAPMP KLATQSNEIT IPVTFESRAQ LGGPEAAKSD
ETAAK

Category

Human HSPB1 ELISA Kit