Human BCL2A1 ELISA Kit

Alternative name
Human bcl-2-like protein 5 ELISA Kit;Human bcl2-L-5 ELISA Kit;Human hemopoietic-specific early response protein ELISA Kit;Human protein BFL-1 ELISA Kit;Human Protein GRS ELISA Kit;Human BCL2L5 ELISA Kit;Human BFL1 ELISA Kit;Human GRS ELISA Kit;Human HBPA1 ELISA Kit;Human ACC-1 ELISA Kit;Human ACC-2 ELISA Kit;Human ACC1 ELISA Kit;Human ACC2 ELISA Kit;Human BCL2 related protein A1 ELISA Kit;Human bcl-2-related protein A1 ELISA Kit;Human hematopoietic BCL2-related protein A1 ELISA Kit;
Catalog
E004530
species
Human
GeneBCL2A1
SpecificityThis assay has high sensitivity and excellent specificity for detection of BCL2A1. No significant cross-reactivity or interference between BCL2A1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BCL2A1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseHuman BCL2A1 ELISA Kit allows for the in vitro quantitative determination of BCL2A1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalPriciple of the assay: BCL2A1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BCL2A1 antibody and an BCL2A1-HRP conjugate. The assay sample and buffer are incubated together with BCL2A1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BCL2A1 concentration since BCL2A1 from samples and BCL2A1-HRP conjugate compete for the anti-BCL2A1 antibody binding site. Since the number of sites is limited, as more sites are occupied by BCL2A1 from the sample, fewer sites are left to bind BCL2A1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BCL2A1 concentration in each sample is interpolated from this standard curve.
Human Bcl-2-related protein A1 Protein information
Uniprot ID B2LA1_HUMAN
Uniprot AC Q16548; Q6FGZ4; Q6FH19; Q86W13; Q99524;
UniGene Hs.227817;
GeneID 597
KEGG hsa:597;
Human Bcl-2-related protein A1 Protein SEQUENCE
SEQUENCE 175 AA; 20132 MW; 329D98AF2BE07A0D CRC64;
MTDCEFGYIY RLAQDYLQCV LQIPQPGSGP SKTSRVLQNV AFSVQKEVEK
NLKSCLDNVN VVSVDTARTL FNQVMEKEFE DGIINWGRIV TIFAFEGILI
KKLLRQQIAP DVDTYKEISY FVAEFIMNNT GEWIRQNGGW ENGFVKKFEP
KSGWMTFLEV TGKICEMLSL LKQYC
UCSC uc002bfc.5; human. [Q16548-1];

Human BCL2A1 ELISA Kit

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