Human OPLAH ELISA Kit

Alternative name
Human 5-oxo-L-prolinase ELISA Kit;Human 5-Opase ELISA Kit;Human pyroglutamase ELISA Kit;Human OPLA ELISA Kit;Human OPLAHD ELISA Kit;Human 5-oxoprolinase (ATP-hydrolysing) ELISA Kit;Human 5-oxoprolinase ELISA Kit;
Catalog
E042111
species
Human
GeneOPLAH
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.094ng/ml
Intended UseHuman OPLAH ELISA Kit allows for the in vitro quantitative determination of OPLAH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human OPLAH antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human OPLAH antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human OPLAH amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human OPLAH can be calculated.
Human 5-oxoprolinase Protein information
Uniprot ID OPLA_HUMAN
Uniprot AC O14841; A5PKY8; Q75W65; Q9Y4Q0;
UniGene Hs.305882;
GeneID 26873
KEGG hsa:26873;
Human 5-oxoprolinase Protein SEQUENCE
SEQUENCE 1288 AA; 137457 MW; 999AC0FA497750F2 CRC64;
MGSPEGRFHF AIDRGGTFTD VFAQCPGGHV RVLKLLSEDP ANYADAPTEG
IRRILEQEAG MLLPRDQPLD SSHIASIRMG TTVATNALLE RKGERVALLV
TRGFRDLLHI GTQARGDLFD LAVPMPEVLY EEVLEVDERV VLHRGEAGTG
TPVKGRTGDL LEVQQPVDLG ALRGKLEGLL SRGIRSLAVV LMHSYTWAQH
EQQVGVLARE LGFTHVSLSS EAMPMVRIVP RGHTACADAY LTPAIQRYVQ
GFCRGFQGQL KDVQVLFMRS DGGLAPMDTF SGSSAVLSGP AGGVVGYSAT
TYQQEGGQPV IGFDMGGTST DVSRYAGEFE HVFEASTAGV TLQAPQLDIN
TVAAGGGSRL FFRSGLFVVG PESAGAHPGP ACYRKGGPVT VTDANLVLGR
LLPASFPCIF GPGENQPLSP EASRKALEAV ATEVNSFLTN GPCPASPLSL
EEVAMGFVRV ANEAMCRPIR ALTQARGHDP SAHVLACFGG AGGQHACAIA
RALGMDTVHI HRHSGLLSAL GLALADVVHE AQEPCSLLYA PETFVQLDQR
LSRLEEQCVD ALQAQGFPRS QISTESFLHL RYQGTDCALM VSAHQHPATA
RSPRAGDFGA AFVERYMREF GFVIPERPVV VDDVRVRGTG RSGLRLEDAP
KAQTGPPRVD KMTQCYFEGG YQETPVYLLA ELGYGHKLHG PCLIIDSNST
ILVEPGCQAE VTKTGDICIS VGAEVPGTVG PQLDPIQLSI FSHRFMSIAE
QMGRILQRTA ISTNIKERLD FSCALFGPDG GLVSNAPHIP VHLGAMQETV
QFQIQHLGAD LHPGDVLLSN HPSAGGSHLP DLTVITPVFW PGQTRPVFYV
ASRGHHADIG GITPGSMPPH STMLQQEGAV FLSFKLVQGG VFQEEAVTEA
LRAPGKVPNC SGTRNLHDNL SDLRAQVAAN QKGIQLVGEL IGQYGLDVVQ
AYMGHIQANA ELAVRDMLRA FGTSRQARGL PLEVSSEDHM DDGSPIRLRV
QISLSQGSAV FDFSGTGPEV FGNLNAPRAV TLSALIYCLR CLVGRDIPLN
QGCLAPVRVV IPRGSILDPS PEAAVVGGNV LTSQRVVDVI LGAFGACAAS
QGCMNNVTLG NAHMGYYETV AGGAGAGPSW HGRSGVHSHM TNTRITDPEI
LESRYPVILR RFELRRGSGG RGRFRGGDGV TRELLFREEA LLSVLTERRA
FRPYGLHGGE PGARGLNLLI RKNGRTVNLG GKTSVTVYPG DVFCLHTPGG
GGYGDPEDPA PPPGSPPQAL AFPEHGSVYE YRRAQEAV
UCSC uc033cce.2; human.;

Category

Human OPLAH ELISA Kit

Human 5-oxo-L-prolinase ELISA Kit;Human 5-Opase ELISA Kit;Human pyroglutamase ELISA Kit;Human OPLA ELISA Kit;Human OPLAHD ELISA Kit;Human 5-oxoprolinase (ATP-hydrolysing) ELISA Kit;Human 5-oxoprolinase ELISA Kit;