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Human SIRT3 ELISA Kit

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  • Alternative name

    Human hSIRT3 ELISA Kit;Human regulatory protein SIR2 homolog 3 ELISA Kit;Human SIR2-like protein 3 ELISA Kit;Human SIR2L3 ELISA Kit;Human sirtuin 3 ELISA Kit;Human NAD-dependent protein deacetylase sirtuin-3, mitochondrial ELISA Kit;Human NAD-dependent deacetylase sirtuin-3, mitochondrial ELISA Kit;Human mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase ELISA Kit;Human silent mating type information regulation 2, S.cerevisiae, homolog 3 ELISA Kit;Human sir2-like 3 ELISA Kit;Human sirtuin type 3 ELISA Kit;
  • Catalog
    E035346
  • species
    Human
  • GeneSIRT3
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human SIRT3. No significant cross-reactivity or interference between human SIRT3 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human SIRT3 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
  • Intended UseHuman SIRT3 ELISA Kit allows for the in vitro quantitative determination of SIRT3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SIRT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SIRT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SIRT3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SIRT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human NAD-dependent protein deacetylase sirtuin-3, mitochondrial Protein information
  • Uniprot ID SIR3_HUMAN
  • Uniprot AC Q9NTG7; B7Z5U6; Q9Y6E8;
  • UniGene Hs.716456;
  • GeneID 23410
  • KEGG hsa:23410;
  • Human NAD-dependent protein deacetylase sirtuin-3, mitochondrial Protein SEQUENCE

  • SEQUENCE 399 AA; 43573 MW; 4BA8BD3AC5FC7901 CRC64;

    MAFWGWRAAA ALRLWGRVVE RVEAGGGVGP FQACGCRLVL GGRDDVSAGL

    RGSHGARGEP LDPARPLQRP PRPEVPRAFR RQPRAAAPSF FFSSIKGGRR

    SISFSVGASS VVGSGGSSDK GKLSLQDVAE LIRARACQRV VVMVGAGIST

    PSGIPDFRSP GSGLYSNLQQ YDLPYPEAIF ELPFFFHNPK PFFTLAKELY

    PGNYKPNVTH YFLRLLHDKG LLLRLYTQNI DGLERVSGIP ASKLVEAHGT

    FASATCTVCQ RPFPGEDIRA DVMADRVPRC PVCTGVVKPD IVFFGEPLPQ

    RFLLHVVDFP MADLLLILGT SLEVEPFASL TEAVRSSVPR LLINRDLVGP

    LAWHPRSRDV AQLGDVVHGV ESLVELLGWT EEMRDLVQRE TGKLDGPDK

  • UCSC uc001lok.5; human. [Q9NTG7-1];



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