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Human DBT ELISA Kit

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  • Alternative name

    Human 52 kDa mitochondrial autoantigen of primary biliary cirrhosis ELISA Kit;Human branched chain 2-oxo-acid dehydrogenase complex component E2 ELISA Kit;Human BCOADC-E2 ELISA Kit;Human branched-chain alpha-keto acid dehydrogenase complex component E2 ELISA Kit;Human BCKAD-E2 ELISA Kit;Human BCKADE2 ELISA Kit;Human dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex ELISA Kit;Human Dihydrolipoamide branched chain transacylase ELISA Kit;Human dihydrolipoyllysine-residue (2-methylpropanoyl)transferase ELISA Kit;Human BCATE2 ELISA Kit;Human E2 ELISA Kit;Human E2B ELISA Kit;Human dihydrolipoamide branched chain transacylase E2 ELISA Kit;Human lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial ELISA Kit;Human BCKAD E2 subunit ELISA Kit;Human E2 component of branched chain alpha-keto acid dehydrogenase complex ELISA Kit;Human branched chain acyltransferase, E2 component ELISA Kit;Human dihydrolipoyl transacylase ELISA Kit;Human lipoamide acyltransferase component of mitochondrial branched-chain alpha-keto acid dehydrogenase complex ELISA Kit;Human mitochondrial branched chain alpha-keto acid dehydrogenase transacylase subunit (E2b) ELISA Kit;
  • Catalog
    E011923
  • species
    Human
  • GeneDBT
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human DBT. No significant cross-reactivity or interference between human DBT and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human DBT is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
  • Intended UseHuman DBT ELISA Kit allows for the in vitro quantitative determination of DBT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DBT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DBT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DBT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DBT bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial Protein information
  • Uniprot ID ODB2_HUMAN
  • Uniprot AC P11182; B2R811; Q5VVL8;
  • UniGene Hs.709187;
  • GeneID 1629
  • KEGG hsa:1629;
  • Human Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial Protein SEQUENCE

  • SEQUENCE 482 AA; 53487 MW; A7CA728C8F33D126 CRC64;

    MAAVRMLRTW SRNAGKLICV RYFQTCGNVH VLKPNYVCFF GYPSFKYSHP

    HHFLKTTAAL RGQVVQFKLS DIGEGIREVT VKEWYVKEGD TVSQFDSICE

    VQSDKASVTI TSRYDGVIKK LYYNLDDIAY VGKPLVDIET EALKDSEEDV

    VETPAVSHDE HTHQEIKGRK TLATPAVRRL AMENNIKLSE VVGSGKDGRI

    LKEDILNYLE KQTGAILPPS PKVEIMPPPP KPKDMTVPIL VSKPPVFTGK

    DKTEPIKGFQ KAMVKTMSAA LKIPHFGYCD EIDLTELVKL REELKPIAFA

    RGIKLSFMPF FLKAASLGLL QFPILNASVD ENCQNITYKA SHNIGIAMDT

    EQGLIVPNVK NVQICSIFDI ATELNRLQKL GSVGQLSTTD LTGGTFTLSN

    IGSIGGTFAK PVIMPPEVAI GALGSIKAIP RFNQKGEVYK AQIMNVSWSA

    DHRVIDGATM SRFSNLWKSY LENPAFMLLD LK

  • UCSC uc001dta.4; human.;



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