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Mouse Gdf5 ELISA Kit

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  • Alternative name

    Mouse Gdf-5 ELISA Kit;Mouse bone morphogenetic protein 14 ELISA Kit;Mouse BMP-14 ELISA Kit;Mouse Bmp14 ELISA Kit;Mouse bp ELISA Kit;Mouse Cdmp-1 ELISA Kit;Mouse brp ELISA Kit;Mouse growth differentiation factor 5 ELISA Kit;Mouse growth/differentiation factor 5 ELISA Kit;Mouse cartilage-derived morphogenetic protein-1 ELISA Kit;
  • Catalog
    E054037
  • species
    Mouse
  • GeneGdf5
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse GDF5. No significant cross-reactivity or interference between Mouse GDF5 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse GDF5 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.094ng/mL
  • Intended UseMouse Gdf5 ELISA Kit allows for the in vitro quantitative determination of Gdf5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GDF5 in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse GDF5. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse GDF5 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GDF5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse GDF5. You can calculate the concentration of Mouse GDF5 in the samples by comparing the OD of the samples to the standard curve.
  • Mouse Growth/differentiation factor 5 Protein information
  • Uniprot ID GDF5_MOUSE
  • Uniprot AC P43027; A2ARK2;
  • UniGene Mm.4744;
  • GeneID 14563
  • KEGG mmu:14563;
  • Mouse Growth/differentiation factor 5 Protein SEQUENCE

  • SEQUENCE 495 AA; 54895 MW; 8FAECD5A9C996D6E CRC64;

    MRLPKLLTLL LWHLAWLDLE LICTVLGAPD LGQRTPGAKP GLTKAEAKER

    PPLARNVFRP GGHIYGVGAT NARAKGSSGQ TQAKKDEPRK MPPRSGGPET

    KPGPSSQTRQ AAARTVTPKG QLPGGKASSK AGSAPSSFLL KKTREPGTPR

    EPKEPFRPPP ITPHEYMLSL YRTLSDADRK GGNSSVKLEA GLANTITSFI

    DKGQDDRGPA VRKQRYVFDI SALEKDGLLG AELRILRKKP LDVAKPAVPS

    SGRVAQLKLS SCPSGRQPAA LLDVRSVPGL DGSGWEVFDI WKLFRNFKNS

    AQLCLELEAW ERGRAVDLRG LGFERTARQV HEKALFLVFG RTKKRDLFFN

    EIKARSGQDD KTVYEYLFSQ RRKRRAPLAN RQGKRPSKNL KARCSRKALH

    VNFKDMGWDD WIIAPLEYEA FHCEGLCEFP LRSHLEPTNH AVIQTLMNSM

    DPESTPPTCC VPTRLSPISI LFIDSANNVV YKQYEDMVVE SCGCR

  • UCSC uc012chg.1; mouse.;



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