SpecificityThis assay has high sensitivity and excellent specificity for detection of human anti-cardiolipin antibody (IgG). No significant cross-reactivity or interference between human anti-cardiolipin antibody (IgG) and analogues was observed.
Intended UseHuman ACA (IgG) ELISA Kit allows for the in vitro quantitative determination of ACA (IgG) , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the Assay||This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Samples are pipetted into the wells with anti-human IgG conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human anti-cardiolipin antibody(IgG) bound in the initial step. The color development is stopped and the intensity of the color is measured.