Human PTN ELISA Kit

Contact Us
  • Alternative name

    Human PTN ELISA Kit;Human heparin-binding brain mitogen ELISA Kit;Human HBBM ELISA Kit;Human heparin-binding growth factor 8 ELISA Kit;Human HBGF-8 ELISA Kit;Human heparin-binding growth-associated molecule ELISA Kit;Human HB-GAM ELISA Kit;Human heparin-binding neurite outgrowth-promoting factor 1 ELISA Kit;Human HBNF-1 ELISA Kit;Human osteoblast-specific factor 1 ELISA Kit;Human OSF-1 ELISA Kit;Human HBNF1 ELISA Kit;Human NEGF1 ELISA Kit;Human HARP ELISA Kit;Human HBGF8 ELISA Kit;Human HBNF ELISA Kit;Human pleiotrophin ELISA Kit;Human heparin affin regulatory protein ELISA Kit;Human heparin-binding neurite outgrowth promoting factor ELISA Kit;Human pleiotrophin (heparin binding growth factor 8, neurite growth-promoting factor 1) ELISA Kit;

  • Catalog
    E029871
  • species
    Human
  • GenePTN
  • Other Species Mouse Ptn ELISA KitBovine PTN ELISA KitChicken PTN ELISA KitPorcine PTN ELISA KitRat Ptn ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of PTN. No significant cross-reactivity or interference between PTN and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PTN and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman PTN ELISA Kit allows for the in vitro quantitative determination of PTN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyNeurobiology
  • Product Description
    specifical
    Intended Uses: This PTN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PTN. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||PTN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PTN antibody and an PTN-HRP conjugate. The assay sample and buffer are incubated together with PTN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PTN concentration since PTN from samples and PTN-HRP conjugate compete for the anti-PTN antibody binding site. Since the number of sites is limited, as more sites are occupied by PTN from the sample, fewer sites are left to bind PTN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PTN concentration in each sample is interpolated from this standard curve.
  • Human Pleiotrophin Protein information
  • Uniprot ID PTN_HUMAN
  • Uniprot AC P21246; Q5U0B0; Q6ICQ5; Q9UCC6;
  • UniGene Hs.371249;
  • GeneID 5764
  • KEGG hsa:5764;
  • Human Pleiotrophin Protein SEQUENCE
  • SEQUENCE 168 AA; 18942 MW; 0359C08B6497B579 CRC64;

    MQAQQYQQQR RKFAAAFLAF IFILAAVDTA EAGKKEKPEK KVKKSDCGEW

    QWSVCVPTSG DCGLGTREGT RTGAECKQTM KTQRCKIPCN WKKQFGAECK

    YQFQAWGECD LNTALKTRTG SLKRALHNAE CQKTVTISKP CGKLTKPKPQ

    AESKKKKKEG KKQEKMLD

  • UCSC uc003vtq.4; human.;



Inquiry About Human PTN ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!