Human NE ELISA Kit

Contact Us
  • Alternative name

    Neutrophil elastase ELISA KIT; Bone marrow serine protease ELISA KIT; Elastase-2 ELISA KIT; Human leukocyte elastase ELISA KIT; HLE ELISA KIT; Medullasin ELISA KIT; PMN elastaseELANE ELISA KIT; ELA2 ELISA KIT; HLE ELISA KIT

  • Catalog
  • species
  • GeneNE
  • Other Species Human NE/ELA2 ELISA KitHuman NE-B ELISA KitMouse NE ELISA KitBovine NA/NE ELISA KitCanine NA/NE ELISA KitChicken NA/NE ELISA KitHuman NA/NE ELISA KitGeneral NA/NE ELISA KitMouse NA/NE ELISA KitPorcine NA/NE ELISA KitRabbit NA/NE ELISA KitRat NA/NE ELISA KitCamel NA/NE ELISA KitGoat NA/NE ELISA KitGuinea Pig NA/NE ELISA KitHamster NA/NE ELISA KitHorse NA/NE ELISA KitMonkey NA/NE ELISA KitSheep NA/NE ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of NE. No significant cross-reactivity or interference between NE and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NE and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman NE ELISA Kit allows for the in vitro quantitative determination of NE , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyMicrobiology
  • Product Description
    Intended Uses: This NE ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human NE. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||NE ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NE antibody and an NE-HRP conjugate. The assay sample and buffer are incubated together with NE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NE concentration since NE from samples and NE-HRP conjugate compete for the anti-NE antibody binding site. Since the number of sites is limited, as more sites are occupied by NE from the sample, fewer sites are left to bind NE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NE concentration in each sample is interpolated from this standard curve.

Inquiry About Human NE ELISA Kit If you hope to order it or contact us directly, please contact us via We will get back to you in 12hrs,Thanks!