Human KISS-54 ELISA Kit

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  • Catalog
  • species
  • GeneKISS-54
  • SpecificityThis kit recognizes natural and recombinant Human KISS-54. No significant cross-reactivity or interference between Human KISS-54 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity37.5pg/mL
  • Intended UseHuman KISS-54 ELISA Kit allows for the in vitro quantitative determination of KISS-54 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human KISS-54 concentrations in serum, plasma and other biological fluids. Principle of the Assay||This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with KISS-54. During the reaction, KISS-54 in the sample or standard competes with a fixed amount of KISS-54 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to KISS-54. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of KISS-54 in the samples is then determined by comparing the OD of the samples to the standard curve.

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