Contact Us
  • Alternative name

    Granulocyte-macrophage colony-stimulating factor ELISA KIT; Colony-stimulating factor ELISA KIT; CSF ELISA KIT; Molgramostin ELISA KIT; SargramostimCSF2 ELISA KIT; GMCSF ELISA KIT; GM-CSF ELISA KIT; CSF ELISA KIT

  • Catalog
  • species
  • GeneGM CSF
  • Standard CurveHuman GM CSF ELISA Kit
  • Other Species Human GM-CSF ELISA KitHuman GM-CSF Ab ELISA KitMouse GM-CSF ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of anti-GM-CSF. No significant cross-reactivity or interference between anti-GM-CSF and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between anti-GM-CSF and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/ml.
  • Intended UseHuman GM CSF ELISA Kit allows for the in vitro quantitative determination of GM CSF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyImmunology
  • Product Description
    Principle of the assay: Anti-GM-CSF ELISA kit applies the competitive enzyme immunoassay technique utilizing a GM-CSF antigen and an anti-GM-CSF-HRP conjugate. The assay sample and buffer are incubated together with anti-GM-CSF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the anti-GM-CSF concentration since anti-GM-CSF from samples and anti-GM-CSF-HRP conjugate compete for the GM-CSF antigen binding site. Since the number of sites is limited, as more sites are occupied by anti-GM-CSF from the sample, fewer sites are left to bind anti-GM-CSF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The anti-GM-CSF concentration in each sample is interpolated from this standard curve.


Inquiry About Human GM CSF ELISA Kit If you hope to order it or contact us directly, please contact us via We will get back to you in 12hrs,Thanks!