Human PPL ELISA Kit

Alternative name
Human 190 kDa paraneoplastic pemphigus antigen ELISA Kit;Human 195 kDa cornified envelope precursor protein ELISA Kit;Human KIAA0568 ELISA Kit;Human periplakin ELISA Kit;
Catalog
E028669
species
Human
GenePPL
SpecificityThis assay has high sensitivity and excellent specificity for detection of human PPL. No significant cross-reactivity or interference between human PPL and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human PPL is typically less than 15.6 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
Intended UseHuman PPL ELISA Kit allows for the in vitro quantitative determination of PPL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PPL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PPL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Periplakin Protein information
Uniprot ID PEPL_HUMAN
Uniprot AC O60437; O60314; O60454; Q14C98;
UniGene Hs.192233;
GeneID 5493
KEGG hsa:5493;
Human Periplakin Protein SEQUENCE
SEQUENCE 1756 AA; 204747 MW; EA7EAF3E756D78B4 CRC64;
MNSLFRKRNK GKYSPTVQTR SISNKELSEL IEQLQKNADQ VEKNIVDTEA
KMQSDLARLQ EGRQPEHRDV TLQKVLDSEK LLYVLEADAA IAKHMKHPQG
DMIAEDIRQL KERVTNLRGK HKQIYRLAVK EVDPQVNWAA LVEEKLDKLN
NQSFGTDLPL VDHQVEEHNI FHNEVKAIGP HLAKDGDKEQ NSELRAKYQK
LLAASQARQQ HLSSLQDYMQ RCTNELYWLD QQAKGRMQYD WSDRNLDYPS
RRRQYENFIN RNLEAKEERI NKLHSEGDQL LAAEHPGRNS IEAHMEAVHA
DWKEYLNLLI CEESHLKYME DYHQFHEDVK DAQELLRKVD SDLNQKYGPD
FKDRYQIELL LRELDDQEKV LDKYEDVVQG LQKRGQQVVP LKYRRETPLK
PIPVEALCDF EGEQGLISRG YSYTLQKNNG ESWELMDSAG NKLIAPAVCF
VIPPTDPEAL ALADSLGSQY RSVRQKAAGS KRTLQQRYEV LKTENPGDAS
DLQGRQLLAG LDKVASDLDR QEKAITGILR PPLEQGRAVQ DSAERAKDLK
NITNELLRIE PEKTRSTAEG EAFIQALPGS GTTPLLRTRV EDTNRKYEHL
LQLLDLAQEK VDVANRLEKS LQQSWELLAT HENHLNQDDT VPESSRVLDS
KGQELAAMAC ELQAQKSLLG EVEQNLQAAK QCSSTLASRF QEHCPDLERQ
EAEVHKLGQR FNNLRQQVER RAQSLQSAKA AYEHFHRGHD HVLQFLVSIP
SYEPQETDSL SQMETKLKNQ KNLLDEIASR EQEVQKICAN SQQYQQAVKD
YELEAEKLRS LLDLENGRRS HVSKRARLQS PATKVKEEEA ALAAKFTEVY
AINRQRLQNL EFALNLLRQQ PEVEVTHETL QRNRPDSGVE EAWKIRKELD
EETERRRQLE NEVKSTQEEI WTLRNQGPQE SVVRKEVLKK VPDPVLEESF
QQLQRTLAEE QHKNQLLQEE LEALQLQLRA LEQETRDGGQ EYVVKEVLRI
EPDRAQADEV LQLREELEAL RRQKGAREAE VLLLQQRVAA LAEEKSRAQE
KVTEKEVVKL QNDPQLEAEY QQLQEDHQRQ DQLREKQEEE LSFLQDKLKR
LEKERAMAEG KITVKEVLKV EKDAATEREV SDLTRQYEDE AAKARASQRE
KTELLRKIWA LEEENAKVVV QEKVREIVRP DPKAESEVAN LRLELVEQER
KYRGAEEQLR SYQSELEALR RRGPQVEVKE VTKEVIKYKT DPEMEKELQR
LREEIVDKTR LIERCDLEIY QLKKEIQALK DTKPQVQTKE VVQEILQFQE
DPQTKEEVAS LRAKLSEEQK KQVDLERERA SQEEQIARKE EELSRVKERV
VQQEVVRYEE EPGLRAEASA FAESIDVELR QIDKLRAELR RLQRRRTELE
RQLEELERER QARREAEREV QRLQQRLAAL EQEEAEAREK VTHTQKVVLQ
QDPQQAREHA LLRLQLEEEQ HRRQLLEGEL ETLRRKLAAL EKAEVKEKVV
LSESVQVEKG DTEQEIQRLK SSLEEESRSK RELDVEVSRL EARLSELEFH
NSKSSKELDF LREENHKLQL ERQNLQLETR RLQSEINMAA TETRDLRNMT
VADSGTNHDS RLWSLERELD DLKRLSKDKD LEIDELQKRL GSVAVKREQR
ENHLRRSIVV IHPDTGRELS PEEAHRAGLI DWNMFVKLRS QECDWEEISV
KGPNGESSVI HDRKSGKKFS IEEALQSGRL TPAQYDRYVN KDMSIQELAV
LVSGQK
UCSC uc002cyd.1; human.;

Category

Human PPL ELISA Kit

Human 190 kDa paraneoplastic pemphigus antigen ELISA Kit;Human 195 kDa cornified envelope precursor protein ELISA Kit;Human KIAA0568 ELISA Kit;Human periplakin ELISA Kit;