Human COQ10B ELISA Kit

Alternative name
Human coenzyme Q10B ELISA Kit;Human coenzyme Q-binding protein COQ10 homolog B, mitochondrial ELISA Kit;Human coenzyme Q10 homolog B ELISA Kit;
Catalog
E009917
species
Human
GeneCOQ10B
SpecificityThis assay has high sensitivity and excellent specificity for detection of Coenzyme Q10 Homolog B (COQ10B). No significant cross-reactivity or interference between Coenzyme Q10 Homolog B (COQ10B) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.118ng/mL.
Intended UseHuman COQ10B ELISA Kit allows for the in vitro quantitative determination of COQ10B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coenzyme Q10 Homolog B (COQ10B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coenzyme Q10 Homolog B (COQ10B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coenzyme Q10 Homolog B (COQ10B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Coenzyme Q10 Homolog B (COQ10B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Coenzyme Q-binding protein COQ10 homolog B, mitochondrial Protein information
Uniprot ID CQ10B_HUMAN
Uniprot AC Q9H8M1; B7Z1Y4;
UniGene Hs.606592; Hs.741586;
GeneID 80219
KEGG hsa:80219;
Human Coenzyme Q-binding protein COQ10 homolog B, mitochondrial Protein SEQUENCE
SEQUENCE 238 AA; 27071 MW; 078ECF9A377908D0 CRC64;
MAARTGHTAL RRVVSGCRPK SATAAGAQAP VRNGRYLASC GILMSRTLPL
HTSILPKEIC ARTFFKITAP LINKRKEYSE RRILGYSMQE MYDVVSGVED
YKHFVPWCKK SDVISKRSGY CKTRLEIGFP PVLERYTSVV TLVKPHLVKA
SCTDGRLFNH LETIWRFSPG LPGYPRTCTL DFSISFEFRS LLHSQLATLF
FDEVVKQMVA AFERRACKLY GPETNIPREL MLHEVHHT
UCSC uc002uuh.2; human. [Q9H8M1-1];

Category

Human COQ10B ELISA Kit

Human coenzyme Q10B ELISA Kit;Human coenzyme Q-binding protein COQ10 homolog B, mitochondrial ELISA Kit;Human coenzyme Q10 homolog B ELISA Kit;