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Alternative name
Kininogen-1 ELISA KIT; Alpha-2-thiol proteinase inhibitor ELISA KIT; Fitzgerald factor ELISA KIT; High molecular weight kininogen ELISA KIT; HMWK ELISA KIT; Williams-Fitzgerald-Flaujeac factorCleaved into the following 6 chains:Kininogen-1 heavy chain ELISA KIT; T-kininAlternative name(s):Ile-Ser-BradykininKNG1 ELISA KIT; BDK ELISA KIT; KNG ELISA KIT; HMWK ELISA KIT
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Catalog
E021877
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species
Human
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GeneKNG1
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Other Species
Mouse Kng1 ELISA KitBovine KNG1 ELISA Kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of human KNG1. No significant cross-reactivity or interference between human KNG1 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe minimum detectable dose of human KNG1 is typically less than 11.75 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
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Intended UseHuman KNG1 ELISA Kit allows for the in vitro quantitative determination of KNG1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KNG1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KNG1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KNG1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KNG1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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