Mouse H2-T3 ELISA Kit

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  • Alternative name

    Mouse MHC thymus leukemia antigen ELISA Kit;

  • Catalog
    E055146
  • species
    Mouse
  • GeneH2-T3
  • Standard CurveMouse H2-T3 ELISA Kit
  • SpecificityThis assay recognizes recombinant and natural mouse H-2 class I histocompatibility antigen, TLA(B) alpha chain. No significant cross-reactivity or interference was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseMouse H2-T3 ELISA Kit allows for the in vitro quantitative determination of H2-T3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to H-2 class I histocompatibility antigen, TLA(B) alpha chain. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for H-2 class I histocompatibility antigen, TLA(B) alpha chain and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain H-2 class I histocompatibility antigen, TLA(B) alpha chain, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of H-2 class I histocompatibility antigen, TLA(B) alpha chain in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse H-2 class I histocompatibility antigen, TLA(B) alpha chain Protein information
  • Uniprot ID HA1T_MOUSE
  • Uniprot AC P14432; P14433; Q31194; Q31203;
  • UniGene Mm.426002;
  • Mouse H-2 class I histocompatibility antigen, TLA(B) alpha chain Protein SEQUENCE
  • SEQUENCE 384 AA; 43479 MW; 305891FC35F41E76 CRC64;

    MRMGTPVPGT LLILLAASQG QTQTCPGSHS LRYFYTALSR PAISEPWYIA

    VGYLDDTQFV RFNSSGETAT YKLSAPWVEQ EGPEYWARET EIVTSNAQFF

    RENLQTMLDY YNLSQNGSHT IQVMYGCEVE FFGSLFRAYE QHGYDGPDYI

    ALNEDLKTWT AADTAAEITR SKWEQAGYTE LRRTYLEGPC KDSLLRYLEN

    RKKTQECTDP PKTHVTHHPR PEGYVTLRCW ALRFYPADIT LTWQLNGEEL

    IQDTELVETR PAGDGTFQKW AAVVVPLGKE QKYTCHVYHE GLPEPLTLRW

    EPPQTSMPNR TTVRALLGAM IILGFMSGSV MMWMRKNNGG NGDDNTAAYQ

    NEREHLSLDP RAESEALGVE AGMKDLPSAP PLVS





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