Human ANG ELISA Kit

Alternative name
Human ribonuclease 5 ELISA Kit;Human RNase 5 ELISA Kit;Human RNASE5 ELISA Kit;Human ALS9 ELISA Kit;Human HEL168 ELISA Kit;Human RAA1 ELISA Kit;Human RNASE4 ELISA Kit;Human angiogenin ELISA Kit;Human angiogenin, ribonuclease, RNase A family, 5 ELISA Kit;Human epididymis luminal protein 168 ELISA Kit;Human ribonuclease A A1 ELISA Kit;Human ribonuclease A family member 5 ELISA Kit;
Catalog
E001993
species
Human
GeneANG
SpecificityNatural and recombinant human ANG
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 12pg/ml
Intended UseHuman ANG ELISA Kit allows for the in vitro quantitative determination of ANG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human ANG ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ANG has been precoated onto 96-well plates. Standards(E.coli, Q25-P147) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ANG is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ANG amount of sample captured in plate. Background: Angiogenin (Ang) also known as ribonuclease 5 is a protein that in humans is encoded by the ANG gene. Angiogenin is a potent stimulator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. Angiogenins are involved in host defense and noted that inflammation provokes upregulated ANG mRNA expression in liver and an increase in detectable ANG protein in serum. The human angiogenin gene was mapped to chromosome 14q11.
Human Angiogenin Protein information
Uniprot ID ANGI_HUMAN
Uniprot AC P03950; Q05CV1; Q53X86; Q6P5T2; Q8WXE7;
UniGene Hs.283749;
GeneID 283
KEGG hsa:283;
Human Angiogenin Protein SEQUENCE
SEQUENCE 147 AA; 16550 MW; 9C462DA3C8D39ACC CRC64;
MVMGLGVLLL VFVLGLGLTP PTLAQDNSRY THFLTQHYDA KPQGRDDRYC
ESIMRRRGLT SPCKDINTFI HGNKRSIKAI CENKNGNPHR ENLRISKSSF
QVTTCKLHGG SPWPPCQYRA TAGFRNVVVA CENGLPVHLD QSIFRRP

Human ANG ELISA Kit

Contact Us