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Alternative name
Beta-2-glycoprotein 1 ELISA KIT; APC inhibitor ELISA KIT; Activated protein C-binding protein ELISA KIT; Apolipoprotein H ELISA KIT; Apo-H ELISA KIT; Beta-2-glycoprotein IApoh ELISA KIT; B2gp1 ELISA KIT; Apo-H ELISA KIT; B2GPI ELISA KIT; Beta(2)GPI ELISA KIT
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Catalog
E045786
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species
Mouse
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Genebeta2-GP
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Standard Curve
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Other Species
Human beta2 GP ELISA KitHuman beta2-GP ELISA Kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of beta2-GP. No significant cross-reactivity or interference between beta2-GP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between beta2-GP and all the analogues, therefore, cross reaction may still exist in some cases.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.1 ng/mL.
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Intended UseMouse beta2-GP ELISA Kit allows for the in vitro quantitative determination of beta2-GP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyMouse ELISA Kit
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Product Description
specificalPrinciple of the assay: beta2-GP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for beta2-GP. Standards or samples are then added to the microtiter plate wells and beta2-GP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of beta2-GP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for beta2-GP are added to each well to "sandwich" the beta2-GP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain beta2-GP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The beta2-GP concentration in each sample is interpolated from this standard curve.
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