SensitivityThe minimum detectable dose of Human Insulin is typically less than 0.5 mU/L.
Intended UseHuman INS ELISA Kit allows for the in vitro quantitative determination of INS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the assay: The Human Insulin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Insulin in Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. This assay employs an antibody specific for Human Insulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Insulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Insulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Insulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background: Insulin is a secreted peptide hormone that elicitsmetabolic effectssuch as increasesin glucose uptake and glycogen synthesisleading to a decrease in blood glucose concentration. Insulin isfirst formed as a precursormolecule, preproinsulin, which islatercleaved to proinsulin and finallyto the mature Insulin hormone.Mature Insulin consists of 51 amino acids,containedwithin an A chain and a B chain that are connected by 2 disulfide bridges. It increases cell permeabilityto monosaccharides, amino acids and fatty acids. Insulin is secreted bythe pancreas at basal levelsin the absence of exogenousstimuli, with secretion increasing in response to glucose. Insulin action is effected by the binding of Insulin to cell-surface receptors on the targetcell membrane. Defects of Insulin are the cause of hyperproinsulinemia and of type-II diabetes mellitus.