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Alternative name
Amyloid beta A4 protein ELISA KIT; ABPP ELISA KIT; APP ELISA KIT; Alzheimer disease amyloid A4 protein homolog ELISA KIT; Amyloidogenic glycoprotein ELISA KIT; AGCleaved into the following 14 chains:N-APPSoluble APP-alpha ELISA KIT; S-APP-alphaSoluble APP-beta ELISA KIT; S-APP-betaC99 ELISA KIT; Alternative name(s): ELISA KIT; APP-C99Beta-amyloid protein 42 ELISA KIT; Alternative name(s): ELISA KIT; Beta-APP42Beta-amyloid protein 40 ELISA KIT; Alternative name(s): ELISA KIT; Beta-APP40C83P3(42)P3(40)C80Gamma-secretase C-terminal fragment 59 ELISA KIT; Alternative name(s): ELISA KIT; APP-C59 ELISA KIT; Amyloid intracellular domain 59 ELISA KIT; AID(59) ELISA KIT; Gamma-CTF(59)Gamma-secretase C-terminal fragment 57 ELISA KIT; Alternative name(s): ELISA KIT; APP-C57 ELISA KIT; Amyloid intracellular domain 57 ELISA KIT; AID(57) ELISA KIT; Gamma-CTF(57)Gamma-secretase C-terminal fragment 50 ELISA KIT; Alternative name(s): ELISA KIT; Amyloid intracellular domain 50 ELISA KIT; AID(50) ELISA KIT; Gamma-CTF(50)App ELISA KIT; APP ELISA KIT; AG ELISA KIT; S-APP-alpha ELISA KIT; S-APP-beta ELISA KIT; AID(59) ELISA KIT; AID(57) ELISA KIT; AID(50) ELISA KIT
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Catalog
E001911
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species
Human
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GeneAbeta1-42
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Standard Curve
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Other Species
Mouse Abeta1-42 ELISA Kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of ?AP1~42. No significant cross-reactivity or interference between ?AP1~42 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ?AP1~42 and all the analogues, therefore, cross reaction may still exist in some cases.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity1.0 pg/mL.
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Intended UseHuman Abeta1-42 ELISA Kit allows for the in vitro quantitative determination of Abeta1-42 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyHuman ELISA Kit
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Product Description
specificalPrinciple of the assay: ?AP1~42 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-?AP1~42 antibody and an ?AP1~42-HRP conjugate. The assay sample and buffer are incubated together with ?AP1~42-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ?AP1~42 concentration since ?AP1~42 from samples and ?AP1~42-HRP conjugate compete for the anti-?AP1~42 antibody binding site. Since the number of sites is limited, as more sites are occupied by ?AP1~42 from the sample, fewer sites are left to bind ?AP1~42-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ?AP1~42 concentration in each sample is interpolated from this standard curve.
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