Mouse PLD ELISA Kit

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  • Alternative name

    Phosphatidylinositol-glycan-specific phospholipase D ELISA KIT; Glycoprotein phospholipase D ELISA KIT; Glycosyl-phosphatidylinositol-specific phospholipase D ELISA KIT; GPI-PLD ELISA KIT; GPI-specific phospholipase DGPLD1 ELISA KIT; PIGPLD1 ELISA KIT; PI-G PLD ELISA KIT; GPI-PLD ELISA KIT; GPI-specific phospholipase D ELISA KIT

  • Catalog
    E063701
  • species
    Mouse
  • GenePLD
  • Standard CurveMouse PLD ELISA Kit
  • Other Species Human PLD ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse PLD ELISA Kit allows for the in vitro quantitative determination of PLD , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyCardiovascular
  • Product Description
    specifical
    Intended Uses: This PLD ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse PLD. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: PLD ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PLD antibody and an PLD-HRP conjugate. The assay sample and buffer are incubated together with PLD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PLD concentration since PLD from samples and PLD-HRP conjugate compete for the anti-PLD antibody binding site. Since the number of sites is limited, as more sites are occupied by PLD from the sample, fewer sites are left to bind PLD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PLD concentration in each sample is interpolated from this standard curve.


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