Human IL6 ELISA Kit

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  • Alternative name

    Human IL-6 ELISA Kit;Human B-cell stimulatory factor 2 ELISA Kit;Human BSF-2 ELISA Kit;Human CTL differentiation factor ELISA Kit;Human CDF ELISA Kit;Human hybridoma growth factor ELISA Kit;Human interferon beta-2 ELISA Kit;Human IFN-beta-2 ELISA Kit;Human IFNB2 ELISA Kit;Human BSF2 ELISA Kit;Human HGF ELISA Kit;Human HSF ELISA Kit;Human interleukin 6 ELISA Kit;Human interleukin-6 ELISA Kit;Human B-cell differentiation factor ELISA Kit;Human interleukin BSF-2 ELISA Kit;

  • Catalog
    E019499
  • species
    Human
  • GeneIL6
  • Standard CurveHuman IL6 ELISA Kit
  • Other Species Mouse Il6 ELISA KitBovine IL6 ELISA KitCanine IL6 ELISA KitGoat IL6 ELISA KitChicken IL6 ELISA KitHorse IL6 ELISA KitMonkey IL6 ELISA KitPorcine IL6 ELISA KitRabbit IL6 ELISA KitRat Il6 ELISA KitSheep IL6 ELISA Kit
  • SpecificityNatural and recombinant human IL-6
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 0.3pg/ml
  • Intended UseHuman IL6 ELISA Kit allows for the in vitro quantitative determination of IL6 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: human IL-6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-6 has been precoated onto 96-well plates. Standards(E.coli, P29-M212) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-6 amount of sample captured in plate. Background: The human interferon-beta 2 gene (IFNB2) product is identical to that for the B-cell stimulation factor-2 (BSF-2), the hybridoma growth factor (HGF) ("interleukin-6"), and the hepatocyte stimulating factor (HSF). Proteins derived from this gene mediate the plasma protein response to tissue injury (acute-phase response) and regulate the growth and differentiation of both B and T cells.1 Interleukin-6 (IL6) has come to be regarded as a potential osteoporotic factorbecause it has stimulatory effects on cells of the osteoclast lineage, and, thus, may play a role in the pathogenesis of bone loss associated with estrogen deficiency.2 IL-6 has many roles essential to the regulation of the immuneresponse, hematopoiesis, and bone resorption.3 It is involved not only in the hepatic acute phase response but alsoin adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion.4 Overproduction of IL-6,a proinflammatory cytokine, is associated with a spectrum of age-related conditions including cardiovascular disease, osteoporosis, arthritis, type 2 diabetes, certain cancers, periodontal disease, frailty, and functional decline.5BSF-2 is a novel interleukin consisting of 184 amino acids.6 The standard product used in this kit is recombinanthuman IL-6, consisting of 184 amino acids with the molecular mass of 20.3kDa.
  • Human Interleukin-6 Protein information
  • Uniprot ID IL6_HUMAN
  • Uniprot AC P05231; Q9UCU2; Q9UCU3; Q9UCU4;
  • UniGene Hs.654458;
  • GeneID 3569
  • KEGG hsa:3569;
  • Human Interleukin-6 Protein SEQUENCE
  • SEQUENCE 212 AA; 23718 MW; 1F1ED1FE1B734079 CRC64;

    MNSFSTSAFG PVAFSLGLLL VLPAAFPAPV PPGEDSKDVA APHRQPLTSS

    ERIDKQIRYI LDGISALRKE TCNKSNMCES SKEALAENNL NLPKMAEKDG

    CFQSGFNEET CLVKIITGLL EFEVYLEYLQ NRFESSEEQA RAVQMSTKVL

    IQFLQKKAKN LDAITTPDPT TNASLLTKLQ AQNQWLQDMT THLILRSFKE

    FLQSSLRALR QM




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