Mouse UCN1 ELISA Kit

Contact Us
  • Catalog
    E072130
  • species
    Mouse
  • GeneUCN1
  • Standard CurveMouse UCN1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Urocortin 1 (UCN1). No significant cross-reactivity or interference between Urocortin 1 (UCN1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 4.35pg/mL.
  • Intended UseMouse UCN1 ELISA Kit allows for the in vitro quantitative determination of UCN1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Urocortin 1 (UCN1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Urocortin 1 (UCN1) and unlabeled Urocortin 1 (UCN1) (Standards or samples) with the pre-coated antibody specific to Urocortin 1 (UCN1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Urocortin 1 (UCN1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Urocortin 1 (UCN1) in the sample.



Inquiry About Mouse UCN1 ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!