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Human KITLG ELISA Kit

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  • Alternative name

    Human mast cell growth factor ELISA Kit;Human MGF ELISA Kit;Human stem cell factor ELISA Kit;Human SCF ELISA Kit;Human c-Kit ligand ELISA Kit;Human sKITLG ELISA Kit;Human DCUA ELISA Kit;Human DFNA69 ELISA Kit;Human FPH2 ELISA Kit;Human FPHH ELISA Kit;Human KL-1 ELISA Kit;Human Kitl ELISA Kit;Human SF ELISA Kit;Human SHEP7 ELISA Kit;Human kit ligand ELISA Kit;Human familial progressive hyperpigmentation 2 ELISA Kit;Human steel factor ELISA Kit;
  • Catalog
    E034311
  • species
    Human
  • GeneKITLG
  • SpecificityNatural and recombinant human SCF
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity Sample For quantitative detection of human SCF in cell culture supernates, serum and plasma
  • Intended UseHuman KITLG ELISA Kit allows for the in vitro quantitative determination of KITLG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: Human SCF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for SCF has been precoated onto 96-well plates. Standards (E Coli, E26-A190) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SCF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human SCF amount of sample captured in plate. Background: Stem Cell Factor (also known as SCF, kit-ligand, KL, or steel factor) is a cytokine that binds to the c-Kit receptor (CD117). It is mapped to 12q21.32. SCF was primarily expressed by perivascular cells throughout the bone marrow, and it plays a role in the regulation of HSCs in the stem cell niche in the bone marrow. SCF can exist both as a transmembrane protein and a soluble protein. This gene plays an important role in the hematopoiesis during embryonic development. During development, the presence of the SCF also plays an important role in the localization of melanocytes, cells that produce melanin and control pigmentation. In addition to it, SCF can promote mast cell adhesion, migration, proliferation, and survival. It also promotes the release of histamine and tryptase, which are involved in the allergic response.
  • Human Soluble KIT ligand Protein information
  • Uniprot ID SCF_HUMAN
  • Uniprot AC P21583; A0AV09; A8K2Q4; B7ZLM4; Q16487; Q68DZ2; Q7M4N8; Q9UQK7;
  • UniGene Hs.1048; Hs.661108;
  • GeneID 4254
  • KEGG hsa:4254;
  • Human Soluble KIT ligand Protein SEQUENCE

  • SEQUENCE 273 AA; 30899 MW; 19FD362CB59C6607 CRC64;

    MKKTQTWILT CIYLQLLLFN PLVKTEGICR NRVTNNVKDV TKLVANLPKD

    YMITLKYVPG MDVLPSHCWI SEMVVQLSDS LTDLLDKFSN ISEGLSNYSI

    IDKLVNIVDD LVECVKENSS KDLKKSFKSP EPRLFTPEEF FRIFNRSIDA

    FKDFVVASET SDCVVSSTLS PEKDSRVSVT KPFMLPPVAA SSLRNDSSSS

    NRKAKNPPGD SSLHWAAMAL PALFSLIIGF AFGALYWKKR QPSLTRAVEN

    IQINEEDNEI SMLQEKEREF QEV

  • UCSC uc001tav.4; human. [P21583-1];



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