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Mouse Cyp46a1 ELISA Kit

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  • Alternative name

    Mouse CH24H ELISA Kit;Mouse cytochrome P450 46A1 ELISA Kit;Mouse Cyp46 ELISA Kit;Mouse cytochrome P450, family 46, subfamily a, polypeptide 1 ELISA Kit;Mouse cholesterol 24-hydroxylase ELISA Kit;Mouse cholestrol 24-hydroxylase ELISA Kit;Mouse cytochrome P450, 46 (cholesterol 24-hydroxylase) ELISA Kit;Mouse cytochrome P450, 46a1 (cholesterol 24-hydroxylase) ELISA Kit;
  • Catalog
    E048307
  • species
    Mouse
  • GeneCyp46a1
  • Standard CurveMouse Cyp46a1 ELISA Kit
  • Other Species Human CYP46A1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CYP46A1. No significant cross-reactivity or interference between CYP46A1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CYP46A1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse Cyp46a1 ELISA Kit allows for the in vitro quantitative determination of Cyp46a1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: CYP46A1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CYP46A1 antibody and an CYP46A1-HRP conjugate. The assay sample and buffer are incubated together with CYP46A1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CYP46A1 concentration since CYP46A1 from samples and CYP46A1-HRP conjugate compete for the anti-CYP46A1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CYP46A1 from the sample, fewer sites are left to bind CYP46A1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CYP46A1 concentration in each sample is interpolated from this standard curve.
  • Mouse Cholesterol 24-hydroxylase Protein information
  • Uniprot ID CP46A_MOUSE
  • Uniprot AC Q9WVK8;
  • UniGene Mm.41911;
  • GeneID 13116
  • KEGG mmu:13116;
  • Mouse Cholesterol 24-hydroxylase Protein SEQUENCE

  • SEQUENCE 500 AA; 56814 MW; F0F178FA65820FF2 CRC64;

    MSPGLLLLGS AVLLAFGLCC TFVHRARSRY EHIPGPPRPS FLLGHLPYFW

    KKDEDCGRVL QDVFLDWAKK YGPVVRVNVF YKTSVIVTSP ESVKKFLMST

    KYNKDSKMYR ALQTVFGERL FGQGLVSECD YGRWYKQRKV MDLAFSRSSL

    VSLMETFNEK AEQLVEILEA KADGQTPVSM QDMLTCATID ILAKAAFGME

    TSMLLGAQKP LSQAVKVMLE GISASRNTLA KFMPGKRKQL REIRESIRLL

    RQVGKDWVQR RREALKRGED MPADILTQIL KAEEGAQDDE VLLDNFVTFF

    IAGHETSANH LAFTVMELSR QPEIVARLQA EVDEVVGSKR HLDYEDLGRL

    QYLSQVLKES LRLYPPAWGT FRLLEEETLI DGVRVPGNTP LLFSTYVMGR

    MDTYFEDPLT FNPDRFGPGA PKPRFTYFPF SLGHRSCIGQ QFAQMEVKVV

    MAKLLQRIEF RLVPGQRFGL QEQATLKPLD PVLCTLRPRG WQPAPPPPPC

  • UCSC uc007ozo.1; mouse.;


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