Human NAGA ELISA Kit

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  • Alternative name

    Human alpha-galactosidase B ELISA Kit;Human D22S674 ELISA Kit;Human GALB ELISA Kit;Human alpha-N-acetylgalactosaminidase ELISA Kit;Human Acetylgalactosaminidase, alpha-N- (alpha-galactosidase B) ELISA Kit;Human N-acetylgalactosaminidase, alpha- ELISA Kit;

  • Catalog
    E001518
  • species
    Human
  • GeneNAGA
  • Standard CurveHuman NAGA ELISA Kit
  • Other Species Mouse Naga ELISA KitBovine NAGA ELISA KitChicken NAGA ELISA KitRat Naga ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of NAGA. No significant cross-reactivity or interference between NAGA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NAGA and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman NAGA ELISA Kit allows for the in vitro quantitative determination of NAGA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyTags
  • Product Description
    specifical
    Principle of the assay: NAGA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NAGA antibody and an NAGA-HRP conjugate. The assay sample and buffer are incubated together with NAGA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NAGA concentration since NAGA from samples and NAGA-HRP conjugate compete for the anti-NAGA antibody binding site. Since the number of sites is limited, as more sites are occupied by NAGA from the sample, fewer sites are left to bind NAGA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NAGA concentration in each sample is interpolated from this standard curve.
  • Human Alpha-N-acetylgalactosaminidase Protein information
  • Uniprot ID NAGAB_HUMAN
  • Uniprot AC P17050;
  • UniGene Hs.75372;
  • GeneID 4668
  • KEGG hsa:4668;
  • Human Alpha-N-acetylgalactosaminidase Protein SEQUENCE
  • SEQUENCE 411 AA; 46565 MW; 781A0728C0B29CD9 CRC64;

    MLLKTVLLLG HVAQVLMLDN GLLQTPPMGW LAWERFRCNI NCDEDPKNCI

    SEQLFMEMAD RMAQDGWRDM GYTYLNIDDC WIGGRDASGR LMPDPKRFPH

    GIPFLADYVH SLGLKLGIYA DMGNFTCMGY PGTTLDKVVQ DAQTFAEWKV

    DMLKLDGCFS TPEERAQGYP KMAAALNATG RPIAFSCSWP AYEGGLPPRV

    NYSLLADICN LWRNYDDIQD SWWSVLSILN WFVEHQDILQ PVAGPGHWND

    PDMLLIGNFG LSLEQSRAQM ALWTVLAAPL LMSTDLRTIS AQNMDILQNP

    LMIKINQDPL GIQGRRIHKE KSLIEVYMRP LSNKASALVF FSCRTDMPYR

    YHSSLGQLNF TGSVIYEAQD VYSGDIISGL RDETNFTVII NPSGVVMWYL

    YPIKNLEMSQ Q

  • UCSC uc003bbw.5; human.;


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