SpecificityThis assay is specific for human IGFBP-2, only low degree of cross reactions was found with dog, horse, donkey, cat and goat. No cross-reactivity was with pig, bovine, rabbit, mouse, chicken, rat, guinea pig, sheep.
SensitivityThe analytical sensitivity of the assay yields 0.2 ng/ml (2x SD of zero standards)
Intended UseHuman IGFBP-2 ELISA Kit allows for the in vitro quantitative determination of IGFBP-2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the assay: The Mediagnost ELISA for IGFBP-2 E05 is a so-called Sandwich-Assay using two specific and high affinity antibodies. The IGFBP-2 in the samples binds to the first antibody coated on the microtiter plate. In the following step the second specific anti-IGFBP-2-Antibody binds in turn to the immobilised IGFBP-2. The second antibody is biotinylated and will be applied in a mixture with a Streptavidin-Peroxidase-Enzyme Conjugate. In the closing substrate reaction the turn of the colour will be catalysed quantitatively depending on the IGFBP-2-level of the samples
Background: Insulin-like growth factors (IGFs) regulate the proliferation, differentation, apoptose, cell adhesion and metabolism in various tissues and cell types. The IGF-I, which is produced mainly in liver under the influence of growth hormone (GH), regulates as hormone the linear growth of the bones and the process of sexual maturity, while IGF-II is mainly a growth factor of fetal tissue (11-13). The biological actions of IGF over the IGF-Type-I receptor are modulated variably through the IGF binding proteins (IGFBP-1 to-6) (14). IGFBP-2 is, after IGFBP-3, the second most frequent IGFBP in the human blood. IGFs, especially tumor typical pro-IGF-forms and hormones regulate the expression of IGFBP-2, GH effect is thereby inhibiting. At cellular level IGFBP-2 seems to stimulate the proliferation and dissemination of solid tumors via an IGF-independent mechanism (15,16).