Mouse Prkaca ELISA Kit

Alternative name
Mouse PKA C-alpha ELISA Kit;Mouse Pkaca ELISA Kit;Mouse PKCD ELISA Kit;Mouse protein kinase, cAMP dependent, catalytic, alpha ELISA Kit;Mouse cAMP-dependent protein kinase catalytic subunit alpha ELISA Kit;Mouse sperm cAMP-dependent protein kinase catalytic subunit ELISA Kit;
Catalog
E046796
species
Mouse
GenePrkaca
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.188ng/ml
Intended UseMouse Prkaca ELISA Kit allows for the in vitro quantitative determination of Prkaca , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse Prkaca antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Prkaca antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Prkaca amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Prkaca can be calculated.
Mouse cAMP-dependent protein kinase catalytic subunit alpha Protein information
Uniprot ID KAPCA_MOUSE
Uniprot AC P05132; Q9JID0;
UniGene Mm.19111;
GeneID 18747
KEGG mmu:18747;
Mouse cAMP-dependent protein kinase catalytic subunit alpha Protein SEQUENCE
SEQUENCE 351 AA; 40571 MW; 02F85D66EB21A1FA CRC64;
MGNAAAAKKG SEQESVKEFL AKAKEDFLKK WETPSQNTAQ LDQFDRIKTL
GTGSFGRVML VKHKESGNHY AMKILDKQKV VKLKQIEHTL NEKRILQAVN
FPFLVKLEFS FKDNSNLYMV MEYVAGGEMF SHLRRIGRFS EPHARFYAAQ
IVLTFEYLHS LDLIYRDLKP ENLLIDQQGY IQVTDFGFAK RVKGRTWTLC
GTPEYLAPEI ILSKGYNKAV DWWALGVLIY EMAAGYPPFF ADQPIQIYEK
IVSGKVRFPS HFSSDLKDLL RNLLQVDLTK RFGNLKNGVN DIKNHKWFAT
TDWIAIYQRK VEAPFIPKFK GPGDTSNFDD YEEEEIRVSI NEKCGKEFTE
F
UCSC uc009mlm.2; mouse. [P05132-2];

Category

Mouse Prkaca ELISA Kit

Mouse PKA C-alpha ELISA Kit;Mouse Pkaca ELISA Kit;Mouse PKCD ELISA Kit;Mouse protein kinase, cAMP dependent, catalytic, alpha ELISA Kit;Mouse cAMP-dependent protein kinase catalytic subunit alpha ELISA Kit;Mouse sperm cAMP-dependent protein kinase catalytic subunit ELISA Kit;