SpecificityTen specificities were tested with concentrations higher than IFNgamma curve concentrations. No cross reaction was observed for concentrations ranging from 2500 to 78.12 pg/ml for IL-1alpha, IL-2, IL-8, IL-12p40, TNFalpha, CD95 Fas, TRAIL, ICAM-1, Gp130 and GM-CSF.
SensitivityThe sensitivity, minimum detectable dose of Human IFNgamma using this Human IFNgamma High Sensitivity ELISA kit was found to be <0.69 pg/ml. This was determined by adding 3 standard deviations to the mean OD obtained when the zero standard was assayed 40
Intended UseHuman IFN gamma ELISA Kit allows for the in vitro quantitative determination of IFN gamma , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Categories/FamilyHuman ELISA Kit
Product Description specificalPrinciple of the Assay: The IFNgamma Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for IFNgamma has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IFNgamma concentrations and unknowns are pipetted into these wells. During the first incubation, the IFNgamma antigen and a biotinylated monoclonal antibody specific for IFNgamma are successively incubated. After washing, the enzyme (streptavidin-peroxydase) is added. All the unbound enzyme is removed by washing and the first amplification step is performed by adding the Biotine-Tyramine reagent. Under the action of HRP, a biotine polymerisation reaction occurs in the region of the HRP linked to the detection antibody. After washing the second amplification step is performed and the polymerised biotine is revealed by a new streptavidin-HRP step. Finally after washing, the substrate is added. The intensity of this coloured product is directly proportional to the concentration of IFNgamma present in the samples.