Human ADORA2A ELISA Kit

Alternative name
Human ADORA2 ELISA Kit;Human A2aR ELISA Kit;Human RDC8 ELISA Kit;Human adenosine A2a receptor ELISA Kit;Human adenosine receptor A2a ELISA Kit;Human adenosine receptor subtype A2a ELISA Kit;
Catalog
E000774
species
Human
GeneADORA2A
Standard Curve
Other Species Mouse Adora2a ELISA Kit Canine ADORA2A ELISA Kit Guinea Pig ADORA2A ELISA Kit Horse ADORA2A ELISA Kit Rat Adora2a ELISA Kit Human ADORA2A-AS1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of ADORA2a. No significant cross-reactivity or interference between ADORA2a and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of ADORA2a is typically less than 0.060ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding
Intended UseHuman ADORA2A ELISA Kit allows for the in vitro quantitative determination of ADORA2A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ADORA2a. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ADORA2a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ADORA2a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ADORA2a in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Adenosine receptor A2a Protein information
Uniprot ID AA2AR_HUMAN
Uniprot AC P29274; B2R7E0;
UniGene Hs.197029;
GeneID 135
KEGG hsa:135;
Human Adenosine receptor A2a Protein SEQUENCE
SEQUENCE 412 AA; 44707 MW; 9438E9D64A6BE61B CRC64;
MPIMGSSVYI TVELAIAVLA ILGNVLVCWA VWLNSNLQNV TNYFVVSLAA
ADIAVGVLAI PFAITISTGF CAACHGCLFI ACFVLVLTQS SIFSLLAIAI
DRYIAIRIPL RYNGLVTGTR AKGIIAICWV LSFAIGLTPM LGWNNCGQPK
EGKNHSQGCG EGQVACLFED VVPMNYMVYF NFFACVLVPL LLMLGVYLRI
FLAARRQLKQ MESQPLPGER ARSTLQKEVH AAKSLAIIVG LFALCWLPLH
IINCFTFFCP DCSHAPLWLM YLAIVLSHTN SVVNPFIYAY RIREFRQTFR
KIIRSHVLRQ QEPFKAAGTS ARVLAAHGSD GEQVSLRLNG HPPGVWANGS
APHPERRPNG YALGLVSGGS AQESQGNTGL PDVELLSHEL KGVCPEPPGL
DDPLAQDGAG VS

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Category

Human ADORA2A ELISA Kit

Human ADORA2 ELISA Kit;Human A2aR ELISA Kit;Human RDC8 ELISA Kit;Human adenosine A2a receptor ELISA Kit;Human adenosine receptor A2a ELISA Kit;Human adenosine receptor subtype A2a ELISA Kit;