SpecificityCross-reactivity. Epidermal growth factor receptor (EGFr) is a closely related molecule to the HER-2/neu growth factor receptor at 88% homology with the intracellular domain and 44% homology with the extracellular domain.
SensitivityMinimal detectable concentration of analyte was determined by repeated measurement of a zero dose sample (Sample Diluent). Mean and standard deviation of apparent recovery of HER-2/neu were calculated for 230 determinations made over a period of 20 days b
Intended UseHuman HER-2/neu ELISA Kit allows for the in vitro quantitative determination of HER-2/neu , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the assay: The HER-2/neu ELISA is a sandwich enzyme immunoassay that utilizes a mouse monoclonal antibody for capture and a different biotinylated mouse monoclonal antibody for the detection of human HER-2/neu protein. Both capture and detector reagents specifically bind to the extracellular domain of HER-2/neu protein. The Capture Antibody has been immobilized on the interior surface of microtiter plate wells. To perform the test, an appropriate volume of specimen is incubated in the coated well to allow binding of the antigen by the Capture Antibody. The immobilized antigen is then reacted with the detector antiserum. The amount of Detector Antibody bound to antigen is measured by binding it with a streptavidin/horseradish peroxidase Conjugate, which then catalyzes the conversion of the chromogenic Substrate o-phenylenediamine (OPD) into a colored product. The colored reaction product is quantitated by spectrophotometry and is related to the amount of HER-2/neu protein in the sample. For instructions, see the Detailed Protocol and Evaluation of Results sections of this booklet