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Catalog
E037814
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species
Human
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GeneBH4
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Human BH4. No significant cross-reactivity or interference between Human BH4 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.78 pg/mL (mean of 6 independent assays).
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Intended UseHuman BH4 ELISA Kit allows for the in vitro quantitative determination of BH4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalIntended Uses: For the quantitative detection of Human Tetrahydrobiopterin (BH4) concentration in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to BH4. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated BH4 and incubated. The competitive inhibition reaction is launched between with HRP labeled BH4 and unlabeled BH4 with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of BH4 in the sample. The color development is stopped and the intensity of the color is measured.
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