Human RPIA ELISA Kit

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  • Alternative name

    Human phosphoriboisomerase ELISA Kit;Human RPI ELISA Kit;Human RPIAD ELISA Kit;Human ribose 5-phosphate isomerase A ELISA Kit;Human ribose-5-phosphate isomerase ELISA Kit;Human ribose 5-phosphate epimerase ELISA Kit;

  • Catalog
    E033665
  • species
    Human
  • GeneRPIA
  • Other Species Mouse Rpia ELISA KitBovine RPIA ELISA KitPorcine RPIA ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.938ng/ml
  • Intended UseHuman RPIA ELISA Kit allows for the in vitro quantitative determination of RPIA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human RPIA antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human RPIA antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human RPIA amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human RPIA can be calculated.
  • Human Ribose-5-phosphate isomerase Protein information
  • Uniprot ID RPIA_HUMAN
  • Uniprot AC P49247; Q541P9; Q96BJ6;
  • UniGene Hs.469264;
  • GeneID 22934
  • KEGG hsa:22934;
  • Human Ribose-5-phosphate isomerase Protein SEQUENCE
  • SEQUENCE 311 AA; 33269 MW; 4ED587A40F10223D CRC64;

    MQRPGPFSTL YGRVLAPLPG RAGGAASGGG GNSWDLPGSH VRLPGRAQSG

    TRGGAGNTST SCGDSNSICP APSTMSKAEE AKKLAGRAAV ENHVRNNQVL

    GIGSGSTIVH AVQRIAERVK QENLNLVCIP TSFQARQLIL QYGLTLSDLD

    RHPEIDLAID GADEVDADLN LIKGGGGCLT QEKIVAGYAS RFIVIADFRK

    DSKNLGDQWH KGIPIEVIPM AYVPVSRAVS QKFGGVVELR MAVNKAGPVV

    TDNGNFILDW KFDRVHKWSE VNTAIKMIPG VVDTGLFINM AERVYFGMQD

    GSVNMREKPF C

  • UCSC uc002ste.4; human.;


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