Intended UseMouse f-Hb ELISA Kit allows for the in vitro quantitative determination of f-Hb , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the Assay||This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hb. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated Hb. The competitive inhibition reaction is launched between with HRP-conjugated Hb and Hb in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of f-Hb in the sample. The color development is stopped and the intensity of the color is measured.