Human FOXO4 ELISA Kit

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  • Alternative name

    Human fork head domain transcription factor AFX1 ELISA Kit;Human AFX ELISA Kit;Human AFX1 ELISA Kit;Human MLLT7 ELISA Kit;Human forkhead box O4 ELISA Kit;Human forkhead box protein O4 ELISA Kit;Human myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 7 ELISA Kit;

  • Catalog
    E015476
  • species
    Human
  • GeneFOXO4
  • Other Species Mouse Foxo4 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human FOXO4. No significant cross-reactivity or interference between Human FOXO4 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human FOXO4 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman FOXO4 ELISA Kit allows for the in vitro quantitative determination of FOXO4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of FOXO4 in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FOXO4. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human FOXO4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FOXO4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human FOXO4. You can calculate the concentration of Human FOXO4 in the samples by comparing the OD of the samples to the standard curve.
  • Human Forkhead box protein O4 Protein information
  • Uniprot ID FOXO4_HUMAN
  • Uniprot AC P98177; B7WPJ7; O43821; Q13720; Q3KPF1; Q8TDK9;
  • UniGene Hs.584654;
  • GeneID 4303
  • KEGG hsa:4303;
  • Human Forkhead box protein O4 Protein SEQUENCE
  • SEQUENCE 505 AA; 53684 MW; 0C71C8E2167CEE68 CRC64;

    MDPGNENSAT EAAAIIDLDP DFEPQSRPRS CTWPLPRPEI ANQPSEPPEV

    EPDLGEKVHT EGRSEPILLP SRLPEPAGGP QPGILGAVTG PRKGGSRRNA

    WGNQSYAELI SQAIESAPEK RLTLAQIYEW MVRTVPYFKD KGDSNSSAGW

    KNSIRHNLSL HSKFIKVHNE ATGKSSWWML NPEGGKSGKA PRRRAASMDS

    SSKLLRGRSK APKKKPSVLP APPEGATPTS PVGHFAKWSG SPCSRNREEA

    DMWTTFRPRS SSNASSVSTR LSPLRPESEV LAEEIPASVS SYAGGVPPTL

    NEGLELLDGL NLTSSHSLLS RSGLSGFSLQ HPGVTGPLHT YSSSLFSPAE

    GPLSAGEGCF SSSQALEALL TSDTPPPPAD VLMTQVDPIL SQAPTLLLLG

    GLPSSSKLAT GVGLCPKPLE APGPSSLVPT LSMIAPPPVM ASAPIPKALG

    TPVLTPPTEA ASQDRMPQDL DLDMYMENLE CDMDNIISDL MDEGEGLDFN

    FEPDP

  • UCSC uc004dys.3; human. [P98177-1];


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